Partial biochemical risk assessment of carbon nanotubes and carbon nanotubes/titanium dioxide nanoparticles on Glyphodes pyloalis (Lepidoptera: Pyralidae)

Volume 9, Issue 4
December 2020
Pages 651-667

Document Type : Original Research

Authors

1 Department of Pesticides Researches, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran.

2 Plant Protection Research Department, Golestan Agricultural and Natural Resources Research and Education Center, AREEO, Gorgan, Iran.

Abstract
Cellular energy allocation (CEA) test was performed in order to investigate the effects and costs of bare carbon nanotubes (CNTs) and CNTs in combination with titanium dioxide nanoparticles (CNTs/TiO2-NPs) on Glyphodes pyloalis Walker after 24, 48 and 72 hours of exposure to 100, 200, 300, 400 and 500 ppm of the treatments. Results showed the negative correlation between total lipid amounts and concentrations of treatments (i.e. CNTs and CNTs/TiO2-NPs) as well as exposure time. Contrary to CNTs treatments, carbohydrate contents were affected by both of CNTs/TiO2-NPs concentration and time of exposure. Results showed that the effect of bare CNTs in the enhancement of glycogen content appeared significantly faster than that of CNTs/TiO2-NPs. Increasing time of exposure to all concentrations of CNTs, except for 100 ppm, prevented enhancement of protein content. The effect of bare CNTs on the reduction of protein contents was faster and greater than that of CNTs/TiO2-NPs. The results indicated that G. pyloalis cannot regulate internal CNTs and CNTs/TiO2-NPs concentrations efficiently without considerable impact on the energy reserves (Ea). The comparison of energy consumed (Ec) in treated larvae showed that CNTs/TiO2-NPs reflected the higher energy demand of the stress response than CNTs. Generally, CEA was significantly decreased as the concentration of CNTs treatments increased. More reduction in CEA amount of all treatments by CNTs/TiO2-NPs than that of the control is also probably considered as a cost to deal with detoxification when the concentration increased and at all the tested time points. Therefore, CEA test might be considered as an early biochemical biomarker for assessing immediate response of organisms after acute exposure to stressors and thus could be applied to risk assessment of nanomaterials.

Keywords

Abega A.V., Ngomo H.M., Nongwe I., Mukaya H.E., Kouoh Sone P.M.A., Yangkou Mbianda X. 2019. Easy and convenient synthesis of CNT/TiO2 nanohybrid by in-surface oxidation of Ti 3+ ions and application in the photocatalytic degradation of organic contaminants in water. Synthetic Metals 251: 1–14.
Adana A., Fen B., Dergisi B., Tunçsoy B.S. 2018. Toxicity of nanoparticles on insects: A Review. Environmental Science and Pollution Research 1(2): 49–61.
Amorim M.J.B., Gomes S.I.L., Soares A.M.V.M., Scott-Fordsmand J.J. 2012. Energy basal levels and allocation among lipids, proteins, and carbohydrates in Enchytraeus albidus: changes related to exposure to Cu salt and Cu nanoparticles. Water, Air, & Soil Pollution 223(1): 477–482.
Bagheri F., Talebi K., Hosseininaveh V. 2010. Cellular energy allocation of pistachio green stink bug, Brachynema germari Kol.(Hemiptera.: Pentatomidae) in relation to juvenoid pyriproxyfen. African Journal of Biotechnology 9(35): 5746-5753.
Bradford M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry72 (1–2): 248–254.
De Coen W.M., Janssen C.R. 1997. The use of biomarkers in Daphnia magna toxicity testing. IV. Cellular energy allocation: a new methodology to assess the energy budget of toxicant-stressed Daphnia populations. Journal of Aquatic Ecosystem Stress and Recovery 6(1): 43–55.
De Coen W.M., Janssen C.R. 2003. The missing biomarker link: Relationships between effects on the cellular energy allocation biomarker of toxicant‐stressed Daphnia magna and corresponding population characteristics. Environmental Toxicology and Chemistry: An International Journal 22(7): 1632–1641.
Elsaesser A., Howard C.V. 2012. Toxicology of nanoparticles. Advanced Drug Delivery Reviews 64 (2): 129–137.
Fouad H., Hongjie L., Hosni D., Wei J., Abbas G., Ga’al H., Jianchu M. 2018. Controlling Aedes albopictus and Culex pipiens pallens using silver nanoparticles synthesized from aqueous extract of Cassia fistula fruit pulp and its mode of action. Artificial Cells, Nanomedicine, and Biotechnology 46(3): 558–567.
Gnaiger E. 1983. Calculation of energetic and biochemical equivalents of respiratory oxygen consumption. p. 337–345. In: "Polarographic oxygen sensors". (E. Gnaiger, H. Forstner, eds.). Polarographic Oxygen Sensors, Springer-Verlag, Berlin, Germany.
Gottschalk F., Nowack B. 2011. The release of engineered nanomaterials to the environment. Journal of Environmental Monitoring 13(5): 1145–1155.
Hardy A., Benford D., Halldorsson T., Jeger M.J., Knutsen H.K., More S., Naegeli Gott D., Oomen A., Weigel S., Karamitrou M., Schoonjans R., Mortensen A. 2011. Guidance on the risk assessment of the application of nanoscience and nanotechnologies in the food and feed chain1. EFSA Journal16(7): 1–36.
Hardy A., Benford D., Halldorsson, T., Jeger M.J., Knutsen H.K., More S., Naegeli Gott D., Oomen A., Weigel S., Karamitrou M., Schoonjans R., Mortensen A. 2018. Guidance on risk assessment of the application of nanoscience and nanotechnologies in the food and feed chain: Part 1, human and animal health. EFSA Journal, 16(7): 5327.
Holmstrup M., Sorensen J.G., Overgaard J., Bayley M., Bindesbol A.M., Slotsbo S., Labouriau R. 2011. Body metal concentrations and glycogen reserves in earthworms (Dendrobaena octaedra) from contaminated and uncontaminated forest soil. Environmental Pollution 159 (1): 190–197.
Institute S.A.S. 2011. SAS/IML 9.3 user’s guide. Sas Institute.
Jackson P., Jacobsen N.R., Baun A., Birkedal R., Kühnel D., Jensen K.A., Wallin H. 2013. Bioaccumulation and ecotoxicity of carbon nanotubes. Chemistry Central Journal 7(1): 1–21.
Jang M., Hwang Y.S. 2018. Effects of functionalized multi-walled carbon nanotubes on toxicity and bioaccumulation of lead in Daphnia magna. Plos One 13(3):1–13.
Kah M., Weniger A.K., Hofmann T. 2016. Impacts of (Nano)formulations on the Fate of an Insecticide in Soil and Consequences for Environmental Exposure Assessment. Environmental Science and Technology 50(20): 10960–10967.
Khalil A.M. 2015. Neurotoxicity and biochemical responses in the earthworm Pheretima hawayana exposed to TiO2-NPs. Ecotoxicology and Environmental Safety 122: 455–461.
Li L., Xu Z., Kah M., Lin D., Filser J. 2019. Nanopesticides: A Comprehensive Assessment of Environmental Risk is Needed before Widespread Agricultural Application. Environmental Science and Technology 53(14): 7923–7924.
Li Z., Gao B., Chen G.Z., Mokaya R., Sotiropoulos S., Li Puma G. 2011. Carbon nanotube/titanium dioxide (CNT/TiO2) core-shell nanocomposites with tailored shell thickness, CNT content and photocatalytic/photoelectrocatalytic properties. Applied Catalysis B: Environmental 110: 50–57.
Memarizadeh N., Ghadamyari M., Adeli M., Talebi-jahromi K. 2014a. Cellular energy allocation of Glyphodes pyloalis (Lep.: Pyralidae): changes related to exposure to TiO2 nanoparticles. Journal of Entomological Society of Iran 33(4): 43–54.
Memarizadeh, N, Ghadamyari, M., Adeli, M. and Talebi, K. 2014b. Biochemical Biomarkers of Glyphodes Pyloalis Walker (Lepidoptera: Pyralidae) in Exposure to TiO2 Nanoparticles. Invertebrate Survival Journal 12: 47–53.
Nguyen M.T., Nguyen C.K., Vu T.M.P., Duong Q. Van Pham T.L., Nguyen T.C. 2014. A study on carbon nanotube titanium dioxide hybrids: Experiment and calculation. Advances in Natural Sciences: Nanoscience and Nanotechnology 5(4): 1–6.
Novais S.C., Soares A.M.V.M., De Coen W., Amorim M.J.B. 2013. Exposure of Enchytraeus albidus to Cd and Zn–Changes in cellular energy allocation (CEA) and linkage to transcriptional, enzymatic and reproductive effects. Chemosphere 90 (3): 1305–1309.
Rai M., Kon K., Ingle A., Duran N., Galdiero S., Galdiero M. 2014. Broad-spectrum bioactivities of silver nanoparticles: the emerging trends and future prospects. Applied Microbiology and Biotechnology 98 (5): 1951–1961.
Rodríguez L.A.A., Pianassola M., Travessa D.N. 2017. Production of TiO2 coated multiwall carbon nanotubes by the sol-gel technique. Materials Research 20: 96–103.
Rueda-Jasso R., Conceiçao L.E.C., Dias J., De Coen W., Gomes E., Rees J.F., Sorgeloos P. 2004. Effect of dietary non-protein energy levels on condition and oxidative status of Senegalese sole (Solea senegalensis) juveniles. Aquaculture 231 (1–4): 417–433.
Schwirn K., Völker D. 2016. Nanomaterials in the environment - Current state of knowledge and regulations on chemical safety: Recommendations of the German Environment Agency 1-36.
Smolders R., De Boeck G., Blust R. 2003. Changes in cellular energy budget as a measure of whole effluent toxicity in zebrafish (Danio rerio). Environmental Toxicology and Chemistry: An International Journal 22(4): 890–899.
Sokolova I.M., Frederich M., Bagwe R., Lannig G., Sukhotin A.A. 2012. Energy homeostasis as an integrative tool for assessing limits of environmental stress tolerance in aquatic invertebrates. Marine Environmental Research 79: 1–15.
Świątek Z.M., Bednarska A.J. 2019. Energy reserves and respiration rate in the earthworm Eisenia andrei after exposure to zinc in nanoparticle or ionic form. Environmental Science and Pollution Research 26 (24): 24933–24945.
Trung T., Cho W.J., Ha C.S. 2003. Preparation of TiO2 nanoparticles in glycerol-containing solutions. Materials Letters 57 (18): 2746–2750.
Van Handel E. 1988. Assay of lipids glycogen and sugars in individual mosquitoes: correlations with length in field-collected Aedes vexans. Journal of The American Mosquito Control Association 4: 549–550.
Venkataraman A., Amadi E.V., Chen Y., Papadopoulos C. 2019. Carbon Nanotube Assembly and Integration for Applications. Nanoscale Research Letters 14 (1): 1–47.
Verslycke T., Roast S.D., Widdows J., Jones M.B., Janssen C.R. 2004. Cellular energy allocation and scope for growth in the estuarine mysid Neomysis integer (Crustacea: Mysidacea) following chlorpyrifos exposure: a method comparison. Journal of Experimental Marine Biology and Ecology 306 (1): 1–16.
Widdows J., Donkin P. 1992. Mussels and environmental contaminants: bioaccumulation and physiological aspects. The Mussel Mytilus: Ecology, Physiology, Genetics and Culture 25: 383–424.
Yuval B., Kaspi R.O.Y., Shloush S., Warburg M.S. 1998. Nutritional reserves regulate male participation in Mediterranean fruit fly leks. Ecological Entomology 23(2): 211–215.