Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae)

Dehghanikhah, Fahimeh; Kazzazi, Majid; Madadi, Hossein; Madadi, Hossein; Hossein Naveh, Vahid

doi:10.48311/jcp.2014.1106

Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae)

10.48311/jcp.2014.1106

Volume 3, Issue 2
June 2014

Pages 181-189

Authors

F. Dehghanikhah ¹

M. Kazzazi ¹

H. Madadi ¹

V. Hossein naveh ²

¹ Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.

² Department of Plant Protection, College of Agriculture, University of Tehran; Karaj, Iran.

Abstract

The Colorado potato beetle, Leptinotarsa decemlineata Say (Col.: Chrysomelidae) is an important pest of potato worldwide and study of its glucosidases is an important step to develop appropriate pest control strategies. In this study, some biochemical aspects of β-glucosidase in the Colorado potato beetle were determined. The results showed that β-glucosidase activity in the midgut of adults was 6.68 Umg^-1. Maximum activity of midgut β-glucosidase occurred at pH 4 to pH 5.5; however, the enzyme is active at pH 3 to pH 7 more than 50% of its relative activity. The enzyme was stable at pH 3 to pH 8 for 2 and 8 hours incubation time. According to the results, optimal temperature for the enzyme activity was 50 °Cand its stability significantly was reduced at 50 °C during 1 to 8 days incubation time. The enzyme activity decreased with the addition of different concentrations of MgCl₂, urea, Tris and CaCl₂. Enzyme activity was highly decreased at low concentrations of SDS (1 mM).

Keywords

Biochemistry

midgut

β-glucosidase

Leptinotarsa decemlineata

20.1001.1.22519041.2014.3.2.3.0

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