Volume 13, Issue 4 (12-2024)
JCP 2024, 13(4): 411-416 |
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Salehzadeh M, Afsharifar A. The first report of melon yellow spot virus Orthotospovirus meloflavi occurrence in Iran. JCP 2024; 13 (4) :411-416
URL: http://jcp.modares.ac.ir/article-3-75770-en.html
URL: http://jcp.modares.ac.ir/article-3-75770-en.html
1- Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, Iran. , mehrdadsalehzadeh@gmail.com
2- Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, Iran.
2- Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, Iran.
Abstract: (199 Views)
During a survey in the spring of 2024, noticeable severe leaf yellow spots, yellowing, fruit necrosis, and chlorotic ring spots symptoms were observed in Cucumber plants cultivated near Isfahan City, central Iran, to assess Orthotospovirus infection. Total genomic RNA was individually extracted from the leaves of 5 symptomatic and one asymptomatic leaf sample. The extracted RNA samples were subsequently subjected to RT-PCR using an Orthotospovirus-universal primer pair. An amplicon of the expected size was obtained with all diseased samples, and a BLAST search of its nucleotide sequences exhibited a high level of nucleotide identity with several MYSP isolates. This is the first report of the melon yellow spot virus (MYSV) Orthotospovirus meloflavi occurrence in Iran.
Article Type: Brief Communication |
Subject:
Plant Viral Diseases
Received: 2024/06/23 | Revised: 2025/02/11 | Accepted: 2024/12/23 | Published: 2025/01/7
Received: 2024/06/23 | Revised: 2025/02/11 | Accepted: 2024/12/23 | Published: 2025/01/7
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