The first report of melon yellow spot virus Orthotospovirus meloflavi occurrence in Iran

Volume 13, Issue 4
December 2024
Pages 411-416

Document Type : Brief Communication

Authors

Plant Virology Research Center, School of Agriculture, Shiraz University, Shiraz, Iran.

Abstract
During a survey in the spring of 2024, noticeable severe leaf yellow spots, yellowing, fruit necrosis, and chlorotic ring spots symptoms were observed in Cucumber plants cultivated near Isfahan City, central Iran, to assess Orthotospovirus infection. Total genomic RNA was individually extracted from the leaves of 5 symptomatic and one asymptomatic leaf sample. The extracted RNA samples were subsequently subjected to RT-PCR using an Orthotospovirus-universal primer pair. An amplicon of the expected size was obtained with all diseased samples, and a BLAST search of its nucleotide sequences exhibited a high level of nucleotide identity with several MYSP isolates. This is the first report of the melon yellow spot virus (MYSV) Orthotospovirus meloflavi occurrence in Iran.




Keywords

Subjects
Bald-Blume, N., Bergervoet, J. H., & Maiss, E. (2017). Development of a molecular assay for the general detection of tospoviruses and the distinction between tospoviral species. Archives of virology, 162, 1519-1528. https://doi.org/10.1007/s00705-017-3256-x.
Deng, D., McGrath, P. F., Robinson, D. J., & Harrison, B. D. (1994). Detection and differentiation of whitefly-transmitted geminiviruses in plants and vector insects by the polymerase chain reaction with degenerate primers. Annals of applied Biology, 125(2), 327-336. https://doi.org/10.1111/j.1744-7348.1994.tb04973.x.
Gibbs, A., & Mackenzie, A. (1997). A primer pair for amplifying part of the genome of all potyvirids by RT-PCR. Journal of Virological Methods, 63(1-2), 9-16. https://doi.org/10.1016/S0166-0934(96)02103-9.
Iwaki, M., Honda, Y., Hanada, K., Tochihara, H., Yonaha, T., Hokama, K., & Yokoyama, T. (1984). Silver mottle disease of watermelon caused by tomato spotted wilt virus. Plant Disease, 68(11), 1006-1008.
Kato, K., Handa, K., & Kameya-Iwaki, M. (2000). Melon yellow spot virus: a distinct species of the genus Tospovirus isolated from melon. Phytopathology, 90(4), 422-426. https://doi.org/10.1094/PHYTO.2000.90.4.422.
Li, Y., Tan, G., Lan, P., Zhang, A., Liu, Y., Li, R., & Li, F. (2018). Detection of tobamoviruses by RT-PCR using a novel pair of degenerate primers. Journal of Virological Methods, 259, 122-128. https://doi.org/10.1016/j.jviromet.2018.06.012.
McLeish, M. J., Zamfir, A. D., Babalola, B. M., Peláez, A., Fraile, A., & García-Arenal, F. (2022). Metagenomics show high spatiotemporal virus diversity and ecological compartmentalisation: Virus infections of melon, Cucumis melo, crops, and adjacent wild communities. Virus Evolution, 8(2), veac095. https://doi.org/10.1093/ve/veac095.
Quito-Avila, D. F., Peralta, E. L., Martin, R. R., Ibarra, M. A., Alvarez, R. A., Mendoza, A., & Ochoa, J. (2014). Detection and occurrence of melon yellow spot virus in Ecuador: an emerging threat to cucurbit production in the region. European journal of plant pathology, 140, 193-197. https://doi.org/10.1007/s10658-014-0454-1
Rojas, M. R., Gilbertson, R. L., Russell, D. R., & Maxwell, D. P. (1993). Use of degenerate primers in the polymerase chain reaction to detect whitefly-transmitted geminiviruses. Plant Disease, 77(4), 340-347. http://dx.doi.org/10.1094/PD-77-0340.
Takeuchi, S., Okuda, M., Hanada, K., Kawada, Y., & Kameya, M. (2001). Spotted wilt disease of cucumber (Cucumis sativus) caused by Melon yellow spot virus. Japanese Journal of Phytopathology, 67(1), 46-51. https://doi.org/10.3186/jjphytopath.67.46.
Takeuchi, S., Shimomoto, Y., & Ishikawa, K. (2009). First report of Melon yellow spot virus infecting balsam pear (Momordica charantia L.) in Japan. Journal of General Plant Pathology, 75(2), 154-156. https://doi.org/10.1007/s10327-009-0143-7.
Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24(8), 1596-1599. https://doi.org/10.1093/molbev/msm092
Wu, H., Liu, M., Li, W., Wang, M., Xiu, J., Peng, B., & Gu, Q. (2024). Development and Application of Droplet Digital PCR Assay for the Detection of Watermelon Silver Mottle Virus and Melon Yellow Spot Virus. Horticulturae, 10(3), 199. https://doi.org/10.3390/horticulturae10030199.