Volume 7, Issue 4 (2018)                   JCP 2018, 7(4): 387-393 | Back to browse issues page

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Nikooei M, Hemmati C. Molecular characterization of a 16SrIX phytoplasma associated with Convolvulus glomeratus witches' broom and with an insect vector in Iran. JCP. 2018; 7 (4) :387-393
URL: http://jcp.modares.ac.ir/article-3-21282-en.html
1- Department of Agriculture, Minab Higher Education Center, University of Hormozgan, Bandar Abbas, Iran.
2- Plant Protection Research Group, University of Hormozgan, Bandar Abbas, Iran. , chamran.hemmati@hormozgan.ac.ir
Abstract:   (3624 Views)
During a survey in 2016, Convolvulus glomeratus showing symptoms resembling those caused by phytoplasmas were observed in Bandar Abbas, Hormozgan province, Iran. These plants were examined for phytoplasma presence by nested-PCR assays using universal primer pair P1/P7 followed by R16F2n/R16R2. All the infected plants indicated positive results using universal primer pairs of P1/P7 followed by R16F2n/R16R2. Results of phylogenetic and virtual RFLP analyses of the 16S rRNA gene sequences revealed that the phytoplasma associated with Convolvulus glomeratus witcheschr('39') broom (CgWB) was a strain of chr('39')Candidatus Phytoplasma phoeniciumchr('39'). The phytoplasma was successfully transmitted to healthy plants by leafhopper, Orosius albicinctus (Distant) which account as a vector of this phytoplasma. This is the first report on the presence of ‘Candidatus Phytoplasma phoenicium’ (phytoplasma group 16SrIX-J) in C. glomeratus and its insect vector in Iran.
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Article Type: Brief Communication | Subject: Prokaryotic Plant Diseases
Received: 2018/05/24 | Accepted: 2018/10/10 | Published: 2018/09/15

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