Changes of phenoloxidase activity in hemocytes of Helicoverpa armigera (Lepidoptera: Noctuidae) exposed to sub-lethal concentrations of insecticides

Zahediasl, Zahra; Farshbaf Pour Abad, Reza; Taghizadeh, Roya; Saber, Moosa

doi:10.48311/jcp.2023.1629

Changes of phenoloxidase activity in hemocytes of Helicoverpa armigera (Lepidoptera: Noctuidae) exposed to sub-lethal concentrations of insecticides

https://doi.org/10.48311/jcp.2023.1629

Volume 12, Issue 2
June 2023

Pages 151-161

Document Type : Original Research

Authors

Z. Zahediasl ¹

R. Farshbaf Pour Abad ¹

R. Taghizadeh ²

M. Saber ¹

¹ Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

² Shahıd Bakeri Higher Educatıon Center, Urmia University, Urmia, Iran.

Abstract

The cotton bollworm, Helicoverpa armigera (Hübner), is one of the most important pests of crops. Like other insects, this insect has an immune system against external threats such as various diseases, parasitoids, and chemical compounds. Phenoloxidase is a key factor in the immunity of insects and other arthropods. The present study investigated the lethal effects of four insecticides, indoxacarb, profenofos, chlorfluazuron, and hexaflumuron, and their effects on phenolic oxidase activity in cotton bollworm. Among the studied insecticides, in two bioassays (mixture of insecticide with artificial food and immersion of sugar beet leaves), Chlorfluazuron had the highest toxicity (LC₅₀ = 1.71 and 3.11 mg ai/liter, respectively). Also, the highest phenol-oxidase activity at 24 h was for larvae treated with chlorfluazuron and hexaflumuron. Also, when treated with chlorfluazuron and hexaflumuron, the larvae fed on an artificial diet had higher phenoloxidase activity than those fed on sugar beet cultivars.

Keywords

Cotton bollworm

immune system

Phenoloxidase enzyme

sugar beet cultivars

20.1001.1.22519041.2023.12.2.3.3

Abisgold, J. D. and Simpson, S. J. 1987. The physiology of compensation by locusts for changes in dietary protein. Journal of Experimental Biology, 129: 329–346.
Adamo, S. A., Jensen, M. and Younger, M. 2001. Changes in lifetime immune-competence in female Gryllus texensis (formerly G. integer): trade-offs between immunity and reproduction. Animal Behaviour, 62: 425-417.
Assar, A. A., El-Mahasen, M.A., Dahi, H. F. and Amin, H. S. 2016. Biochemical effects of some insect growth regulators and bio-insecticides against cotton leaf-worm, Spodoptera littoralis (Boisduval, 1833.) (Lepidoptera: Noctuidae). Journal of Bioscience and Applied Research, 8: 582-589.
Azambuja, P. D., Garcia, E. S. and Ratcliffe, N. A. 1991. Aspects of classification of Hemiptera hemocytes from six triatomine species. Memorias do Instituto Oswaldo Cruz, 86(1): 1-10.
Bakr, R., El-Monairy, O. and El-barky, N. 2010. Toxicological and behavioral effects of chlorfluazuron on pheromone production and perception of Tribolium castaneum (Coleoptera: Tenebrionidae). Journal of Biological Sciences, 2(2): 61-72.
Bradford, M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-245.
El- Wakell, N. M. E. 2003. New aspect of biological control of Helicoverpa armigera in organic cotton production. Ph.D. Thesis, Goettingen, Germany, 140 pp.
Fitt, G. P. 1989. The ecology of Heliothis species in relation to agro-ecosystems. Annual Review of Entomology, 34(1): 17-53.
Gillespie, J. P., Burnett, C. and Charnley, A. K. 2000. The immune response of the desert locust Schistocerca gregaria during mycosis of the entomopathogenic fungus, Metarhizium anisopliae var acridum. Journal of Insect Physiology, 46: 429-437.
Gillespie, J. P., Kanost, M. R. and Trenczeck, T. 1997. Biological mediators of insect immunity. Annual Review of Entomology, 42: 611-643.
Lavine, M. and Strand, M. 2002. Insect hemocytes and their role in immunity. Insect Biochemistry and Molecular Biology, 32: 1295-1309.
Mirhaghparast, S. K., Zibaee, A., Jalali-Sendi, J., Hoda, H. and Fazeli-Dinan, M. 2015. Immune and metabolic responses of Chilo suppressalis Walker (Lepidoptera: Crambidae) larvae to an insect growth regulator, hexaﬂumuron. Pesticide Biochemistry and Physiology, 125: 69-77.
Moadeli, T., Hejazi, M. J. and Golmohammadi, G. 2014. Lethal effects of pyriproxyfen, spinosad, and indoxacarb and sublethal effects of pyriproxyfen on the 1st instar larvae of beet armyworm, Spodoptera exigua Hübner (Lepidoptera: Noctuidae) in the Laboratory. Journal of Agricultural Science and Technology, 16: 1217-1227.
Rafiei, Dastjerdi, H., Hejazi, M. J., Nouri, G. G. and Saber, M. 2008. Toxicity of some biorational and conventional insecticides to cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae) and its ectoparasitoid, Habrobracon hebetor (Hymenoptera: Braconidae). Journal of Entomological Society of Iran, 28(1): 27-37.
Rahimi, V., Zibaee, A., Mojahed, S., Maddahi, K. and Zare, D. 2013. Effects of pyriproxyfen and hexaflumuron on cellular immunity of Ephestia kuehniella Zeller (Lepidoptera: Pyralidae). Romanian Journal of Biology-Zoology, 58(2): 151-162.
Srygley, R. B., Lorch, P. D., Simpson, S. J. and Sword, G. A. 2009. Immediate protein dietary effects on movement and the generalized immunocompetence of migrating Mormon crickets Anabrus simplex (Orthoptera: Tettigoniidae). Ecological Entomology, 34: 663-668.
Taha, H. S. and Al-Hadek, M. K. 2017. Biochemical studies of chlorfluazuron and diflubenzuron effect on Chitinase and phenoloxidase and biological studies on the black cutworm Agrotis ipsilon (HUFN.) (Lepidoptera: Noctuidae). Journal of Agricultural Chemistry and Biotechnology, 8: 143-148.
Taleh, M., Pourabad, R. F., Geranmaye, J. and Ebadollahi, A. 2015. Toxicity of Hexaflumuron as an insect growth regulator (IGR) against Helicoverpa armigera Hubner (Lepidoptera: Noctuidae). Journal of Entomology and Zoology Studies, 3(2): 274-277.
Zibaee, A., Bandani, A. R. and Malagoli, D. 2011. Purification and characterization of phenoloxidase from the hemocytes of Eurygaster integriceps (Hemiptera: Scutelleridae). Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 158: 117-123.
Zibaee, A., Bandani, A. R., Talaei‐Hassanlouei, R. and Malagoli, D. 2009. Temperature and Ca2+ ion as modulators in cellular immunity of the Sunn pest Eurygaster integriceps Puton (Heteroptera: Scutelleridae). Entomological Research, 39: 364-37.

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