Population genetic structure of Ustilago maydis in Iran

Momeni, Hassan; Nazari, Fahimeh

doi:10.48311/jcp.2022.1612

Population genetic structure of Ustilago maydis in Iran

10.48311/jcp.2022.1612

Volume 11, Issue 4
December 2022

Pages 495-507

Document Type : Original Research

Authors

H. Momeni

F. Nazari

Plant Disease Research Department, Iranian Research Institute of Plant Protection (IRIPP), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran.

Abstract

The fungus Ustilago maydis causes common smut disease in corn. Under favorable conditions, it can cause severe damage to corn. In this study, the genetic structure of U. maydis populations in Iran from the most corn-growing regions of seven provinces, including Ardebil, Fars, Isfahan, Kerman, Kermanshah, Khuzestan, and Qazvin, was evaluated using rep-PCR with primers; BOX, ERIC, and REP. Rep-PCR reactions with 109 isolates of U. maydis produced seven distinct clusters consistent with their geographical origin with few exceptions. The results of AMOVA revealed significant genetic differences within and between pathogen populations. The Euclidean similarity coefficient and the UPGMA algorithm indicate five distant clusters based on the disease severity index. The mean comparison of the disease severity index grouped target isolates into 18 clades using the Tukey test. Our findings showed that the pathogenicity assay-based grouping was not consistent with those of the geographical origin of the isolates nor their genetic similarity.

Keywords

Corn

common smut

Pathogenicity

Genetic diversity

20.1001.1.22519041.2022.11.4.6.3

Subjects

Population Genetics of Plant Pathogens

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