Influence of wheat cultivars on digestive enzyme activity and protein content of the Sunn pest, Eurygaster integriceps (Hem.: Scutelleridae)

Ghanbari, Solmaz; Farshbaf Pourabad, Reza; Ashouri, Shabnam

doi:10.48311/jcp.2022.1582

Influence of wheat cultivars on digestive enzyme activity and protein content of the Sunn pest, Eurygaster integriceps (Hem.: Scutelleridae)

https://doi.org/10.48311/jcp.2022.1582

Volume 11, Issue 1
March 2022

Pages 107-119

Document Type : Original Research

Authors

S. Ghanbari ¹

R. Farshbaf Pourabad ¹

S. Ashouri ²

¹ Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.

² Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Karaj, Iran.

Abstract

The Sunn pest, Eurygaster integriceps (Puton) (Hem.: Scutelleridae), is an economic pest of wheat that reduces the quantitative and qualitative properties of wheat products. We investigated the effect of E. integriceps feeding on six different wheat cultivars (Ghermez (Red), Noodle, Antanius, Sabalan, Azar 2 (with two types of cultivation), and Sardari) on the protein content in the adult’s gut and fat body and their digestive enzymatic activity. All qualified values of the insect feeding on wheat cultivars differed significantly. The least amount of adult weight and protein content of gut and fat body were observed in the insects fed on Ghermez (Red) cultivar, and the highest amount belonged to the Sardari cultivar. The same results for protein content were obtained from SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Also, the lowest and highest gut α-amylase, pectinase, and protease activities were in Ghermez and Sardari cultivars, respectively. Therefore, it was concluded that the type of wheat cultivar affects the food preference of this insect and, thereby, physiological parameters of the insect gastrointestinal tract. Planting a wheat cultivar like “Ghermez,” which may be resistant, can be a suitable and cost-effective method to decrease the chemicals applied against this pest.

Keywords

α-amylase

Pectinase

protease

SDS-PAGE

Sunn pest

20.1001.1.22519041.2022.11.1.9.0

Abdolahadi, F., Kazazi, M. and Najafi Mirak, T. 2016a. Inhibition of Eurygaster integriceps digestive α-amylase by enzymes inhibitors of the wheat varieties; Arvand, Behrang and Hirmand. Agricultural biotechnology, 7(1): 35-43. (in Persian)
Abdolahadi, F., Mirmoayedi, A. and Seifikar, M. 2016b. Inhibition of digestive α-amylase from Eurygaster integriceps by a proteinaccous extract from wheat varieties Bezostaya, Gaspard and Darab. Journal of Fundamental and Applied Science, 8(2): 1759-1770.
Ali, L., El Bouhssini, M., Al-Salti, N., Valkoun, V., Nachit, M., Abdallah, O. and Singh, M. 2009. Evaluation of some wheat and its wild relative accessions for resistance to Sunn pest (Eurygaster integriceps Put.). Arab Journal Plant Protection, 27: 10–13.
Asgari, H. 2017. Instructions for Biological Control of Sunn Pest in Over-wintering Places. Iranian Plant Protection Research Institute. Agricultural Research, Education and Extension Organization.
Ashouri, S., Pourabad, R.F., Zihnioglu, F. and Kocazorbaz, E.K. 2017. Influence of red kidney bean seed proteins on development, digestive α-amylase and gut protein pattern of Leptinotarsa decemlineata (Say). Journal of the Entomological Research Society, 19(3): 69-83.
Bandani, A. 2013. Insect Physiology. University of Tehran Press.
Berlinger, M.J., Tamim, M., Tal, M. and Miller A.R. 1997. Resistance mechanisms of Lycopersicon pennellii accession to Spodoptera littoralis (Lep.: Noctuidae). Journal of Economic Entomology, 90: 1690-1696.
Bernfeld, P. 1955. Amylase, α and β. Methods in Enzymology, 1: 149-158.
Boyd, D.W., Cohen, A.C. and Alverson, D.R. 2002. Digestive enzymes and stylet morphology of Deraeocoris nebulosus (Hemiptera: Miridae), a predacious plant bug. Annals of the Entomological Society of America, 95: 395-401.
Bradford, B.B. 1976. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Annals of Clinical Biochemistry, 72(1-2): 248-254.
Canavoso, L.E., Jouni, Z.E., Karnas, K.J., Pennington, J.E. and Wells, M.A., 2001. Fat metabolism in insects. Annual Review of Nutrition, 21(1): 23-46.
Cato, L. and Mullan, D. 2020. Breeding, selection and quality of partial waxy wheat. Cereal Foods World, 65(5), https://doi.org/10.1094/CFW-65-5-0054.
Cohen, A.C. 2000. How carnivorous bugs feed, 563-570, In C. W. Schaefer and A. R. Panizzi (Eds.), Heteroptera of Economic Importance. CRC, Boca Raton, FL.
Critchley, B.R. 1998. Literature review of sunn pest Eurygaster integriceps Put. (Hemiptera, Scutelleridae). Crop Protection, 17(4): 271-287.
Davari, A. and Parker, B.L. 2018. A review of research on sunn pest, Eurygaster integriceps put. (Hemiptera, Scutelleridae). Journal of Asia Pacific Entomology, 21(1): 352-360.
El Bouhssini, M., Street, K., Joubi, A., Ibrahim, Z. and Rihawi, F. 2009. Sources of wheat resistance to Sunn pest, Eurygaster integriceps Puton, in Syria. Genetic Resources and Crop Evolution, 56(8): 1065-1069.
Elpidina, E.N., Vinokurov, K.S., Gromenko, V.A., Rudenskay, Y.E. and Zhuzhikov, D.P. 2001. Compartmentalization of proteinases and amylases in Nauphoeta cinereal L. midgut. Insect Biochemistry and Physiology, 48: 206-216.
Estela, L., Arrese, L. and Jose, L. 2010. Insect fat body: Energy, metabolism and regulation. Annual Review of Entomology, 55: 207-225.
Farhoudi, N., Kazzazi, M., Hosseininaveh, V. and Arezi, L. 2019. Inhibitory effect of proteinaceous seed extract of three Iranian wheat cultivars on Eurygaster integriceps digestive enzymes. Archives of Phytopathology and Plant Protection, 52(15-16): 1177-1192.
Gatehouse, A.M., Norton, E., Davison, G.M., Babbe, S.M., New, C.A. and Gatehouse, J.A. 1999. Digestive proteolytic activity in larvae of tomato moth, Lacanobia oleracea (Lep.: Noctuidae): effects of plant protease inhibitors in vitro and in vivo. Journal of Insect Physiology, 45(6): 545-585.
Haghparast, R. 2013. Principles of Dryland Wheat Cultivation Mixed with New and Local Knowledge. Agricultural Education and Extension Publications.
Hossieni Migan, H., Derakhshan Shadmehri, A., Shirazi, J. and Hakimi Tabar, M. 2019. Investigation of some biological parameters of Schizaphis graminum (Rodani) on some wheat cultivars in Miami area. M.Sc. thesis. Shahrood University of Technology. Agricultural Engineering. Semnan, 253 pp.
Kinaji, E. and Kinaji, G. 2004. Quality and yield losses due to Sunn pest, Eurygaster integriceps (Hemiptera, Scutelleridae) in different wheat types in Turkey. Field Crops Research, 89(2-3): 187-197.
Laemmli, U.K. 1970. Cleavage of structural protein during the assembly of the head of bacteriophage. Nature, 221: 680-685.
Lazarević, J. and Janković‐Tomanić, M., 2015. Dietary and phylogenetic correlates of digestive trypsin activity in insect pests. Entomologia Experimentalis et Applicata, 157(2): 123-151.
Lehninger, A. 1975. Biochemistry: the molecular basis of cell structure and function. Worth Publishers.
Malihipour, A., Esmailzadeh Mogaddam, M., Najafiyan, G., Roustai, M., Najafi Mirak, T., Amini, A., Khodarahmi, M. and Bakhtiyar, F., 2021. Iranian wheat cultivars (introduced during 1932-2019). Seed and plant breeding research institute.
Mikhailova, N. 1983. Model of a wheat variety resistance to sucking pests. :union: of Sovient Socialist Biological Science, 2: 32-35.
Mohammadzadeh V., Farshbaf Pourabad R., Ashouri S. and Hosseini N. 2020. Impact of four diets on pupal and larval total protein and digestive α-amylase activity in Ephestia kuehniella (Lepidoptera: Pyralidae). Journal of Crop Protection, 9 (4) :615-624.
Mostafavi, Kh., Hosseinzadeh, A., Zeinali, H. and Bagheri, M. 2005. Genetic study of Sunn pest resistance in bread wheat. Iranian Agricultural Sciences, 36(2): 341-351. (in Persian)
Nejat, S., Farshbaf Pour Abad., R. and Ashouri, SH. 2020. Impact of different diets on some biological and physiological parameters in the Helicoverpa armigera (Lepidoptera: Noctuidae). Applied Research in Plant Protection, 9(2): 61-74. (in Persian)
Nasrollahi, S., Badakhshan, H. and Sadeghi, A. 2019. Analyzing Sunn pest resistance in bread wheat genotypes using phenotypic characteristics and molecular markers. Physiology and Molecular Biology of Plants, 25(3): 765-778.
Rezabeigi, M., Esmaili, M., Nouri-Ganbalani, G., Abdollahi, G.A. and Heidari, R. 2000. Investigation on resistance mechanism of wheat cultivars to the sunn pest, Eurygaster integriceps Put. based on HMW-glutenin subunits and measurement of starch granules in kernel endosperm. Ph.D. thesis. Islamic Azad University. Science and Research Branch. Tehran, 253 pp.
Saadati, F. and Bandani, A.R. 2011. Effects of serine protease inhibitors on growth and development and digestive serine proteinases of the sunn pest, Eurygaster integriceps. Journal of Insect Science, 11(1): 72.
Sivri, D., Sapirstein, H.D., Bushuk, W. and koksel, H. 2002. Wheat intercultivar difference in susceptibility of glutenin protein to effects of bug (Eurygaster integriceps) protease. Cereal Chemistry, 79(1): 41-44.
Swingle, H.S. 1925. Digestive enzymes of an insect. The Ohio Journal of Science, 25(5): 34-40.
Wool, D., Namir, Z. and Bergerson, O. 1986. Dietary regulation of amylase activity levels in Tribolium castaneum (Coleoptera: Tenebrionidae) :(Tribolium). Annals of the Entomological Society of America, 79(3): 407-413.
Yazdani, E., Jalali Sendi, J., Zibaee, A. and Ghadamyari, M. 2010. Enzymatic properties of α-amylase in the midgut and salivary glands of mukberry moth, Glyphodes pyloalis Walker (Lepidoptera: Pyralidae). Comptes Rendus Biologies, 333(1): 17-22.

XML

PDF 757.27 K