Application of plant growth-promoting rhizobacteria to protect bell pepper against Tobacco mosaic virus

Aeini, Milad; Ghodum Parizipour, Mohamad Hamed; Eftekhari, Seyed Abdollah; Pooladi, Parnian

doi:10.48311/jcp.2021.1569

Application of plant growth-promoting rhizobacteria to protect bell pepper against Tobacco mosaic virus

https://doi.org/10.48311/jcp.2021.1569

Volume 10, Issue 4
December 2021

Pages 711-722

Document Type : Original Research

Authors

M. Aeini ¹

M. H. Ghodum Parizipour ²

S. A. Eftekhari ³

P. Pooladi ¹

¹ Department of Plant Protection, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

² Department of Plant Protection, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Iran.

³ Department of Horticulture, Faculty of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Abstract

Tobacco mosaic virus (TMV) is one of the economically important plant viruses which causes disease in various crops throughout the world. It has been reported that plant growth-promoting rhizobacteria (PGPR) can be used as potential biocontrol agents against plant viruses. Herein, greenhouse experiments were conducted to undertake the trilateral interactions among PGPR, bell pepper, and TMV. To this end, four-leaf-stage bell pepper seedlings were pre-treated by the PGPR, including Pseudomonas fluorescens, P. putida, and Bacillus subtilis in single and multiple application methods. The plants were then mechanically inoculated with TMV and visually inspected for symptom development till 28 days post-inoculation (dpi). The TMV accumulation in inoculated plants was quantitatively measured by Indirect-ELISA 28 dpi. Analysis of the extinction values showed that application of the PGPR was associated with the least significant (p < 0.05) value (0.08) compared to the positive control (0.77). Inoculation of PGPR triggered the biosynthesis of the defense-related enzymes such as catalase, peroxidase, ascorbate peroxidase, and superoxide dismutase, mediating the biochemical protection against TMV in bell pepper plants. In addition to the disease control, a significant (p < 0.05) increase in growth parameters was observed in PGPR-treated plants compared to the control plants. In conclusion, these results indicated that multiple applications of PGPR strains enhanced the plant vigor and provided an increased level of TMV suppression in bell pepper plants.

Keywords

PGPR

Indirect-ELISA

virus accumulation

Enzyme

20.1001.1.22519041.2021.10.4.11.1

Subjects

Biocontrol of Plant Diseases

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