Journal of Crop Protection

Identification of bean root rot casual and associated fungal agents in Khomein county, Markazi province, Iran

https://doi.org/10.48311/jcp.2021.1563
Volume 10, Issue 4
December 2021
Pages 633-646
Journal of Crop Protection

Document Type : Original Research

Authors

G. Sepehri 1
S. Mojerlou 2
S. Shahbazi 3

1 Department of Plant Biotechnology, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran.

2 Department of Horticulture and Plant Protection, Faculty of Agriculture, Shahrood University of Technology, Shahrood, Iran.

3 Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute (NSTRI), Atomic Energy Organization of Iran (AEOI), Alborz, Iran.

Abstract
Beans are major feed crops belonging to the family Leguminosae, representing one of the most consumed legumes in Iran. Root rot diseases account for high yield losses in bean crops annually, driving the need to identify causative pathogenic agents. During the cropping season of 2019, samples were collected from rhizosphere and infected plant tissues in Khomein county. Of the total 80 purified isolates, 19 fungal isolates were selected for morphological and molecular identification studies. Fusarium equiseti, Fusarium acuminatum, and Macrophomina phaseolina were isolated from the infected crown and root tissues. Their pathogenicity on bean cultivars (Yaghout, Dorsa, and Koosha) is reported for the first time from Markazi province. However, the pathogenicity of fifteen saprophytic and pathogenic fungi species that are isolated from the rhizosphere needs to be confirmed in further studies. Disease symptoms were found to be more severe on pinto beans than red and white beans. In all cases, molecular studies using ITS and tef genomic region confirmed the morphological results. Briefly, these fungal species are introduced as bean root rot causal agents in Khoemin county, which needs to be considered in upcoming breeding programs.

Keywords

Bean
Fusarium spp
Molecular Identification
root and crown rot
Subjects
Ahari Mostafavi, H. 2009. Evaluation of the possibility of biological control of bean root rot disease using non-pathogenic mutants of Fusarium solani f. sp. phaseoli. Journal of Plant Production Research, 16:135-149.
Ahari Mostafavi, H., Mirmajlessi, S. M., Safaie, N., Minassyan, V., Fathollahi, H., Dorri, H. R. and Mansouripour, S. M. 2012. The use of a gamma-irradiated mutants of F. solani f. sp. phaseoli with reduced pathogenicity for the biological control of fusarium root rot of bean (Phaseolus vulgaris) in field conditions. Journal of Agricultural Science and Technology, 14: 1415-1423.
Ahmadi, K., Ebadzadeh, H., Hatami, F., Abdeshah, H. and Kazemian, A. 2019. Agricultural statistics of the crop year 2017-2018: Volume 1: crops. Ministry of Agriculture Jihad Publications, Tehran, Iran.
Alagheh Bandzadeh, N. 2007. Pathogenic diversity and genetic diversity of Macrophomina phaseolina isolates. Master thesis, Faculty of Agriculture, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Aoki, T., O’Donnell, K. and Scandianim, M. M. 2005. Sudden death syndrome of soybean in South America is caused by four species of Fusarium: Fusarium brasiliense sp. nov., F. cuneirostrum sp. nov., F. tucumaniae, and F. virguliforme. Mycoscience, 46:162–183.
Aoki, T., O’Donnell, K., Homma, Y. and Lattanzi, A. R. 2003. Sudden-death syndrome of soybean is caused by two morphologically and phylogenetically distinct species within the Fusarium solani species complex—F. virguliforme in North America and F. tucumaniae in South America. Mycologia, 95: 660-684.
Asan, A. 2011. Checklist of Fusarium species reported from Turkey. Mycotaxon, 116: 479.
Azimi, S., Farokhi Nezhad, R. and Mousavi Jorf, S. A. 2005. Identification and Study on pathogenicity of Fusarium spp. associated with foot and root of Faba Bean in Khuzestan. Scientific Journal of Agriculture, 28: 149-164.

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