Incidence and molecular characterization of Beet virus Q in sugar beet production areas of Iran based on coat protein gene

Moradi, Zohreh; Mehrvar, Mohsen

doi:10.48311/jcp.2021.1556

Incidence and molecular characterization of Beet virus Q in sugar beet production areas of Iran based on coat protein gene

https://doi.org/10.48311/jcp.2021.1556

Volume 10, Issue 3
September 2021

Pages 557-564

Document Type : Original Research

Authors

Z. Moradi ¹

M. Mehrvar ²

¹ Department of Plant Pathology, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

² Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

Beet virus Q (BVQ) is a soilborne pomovirus (family Virgaviridae) associated with rhizomania syndrome in sugar beet. In the present study, BVQ was investigated in sugar beet farms of 12 provinces in Iran by RT-PCR. Thirty-five out of 214 root samples resulted in a positive reaction to BVQ (16.3%). Moreover, 501-bp- long fragments of the coat protein gene of 11 Iranian isolates were purified, cloned, and sequenced. Phylogenetic analysis using the 501 bp fragment of 17 BVQ isolates (11 from this study and six retrieved from GenBank) showed that all isolates clustered in two main groups. Iranian isolates belonged to group I alongside isolates from France (AJ810289) and Germany (AJ223597). Iranian isolates shared 98.80–100% nucleotide sequence identity (98.19–100% amino acid identity) and 97.21–99.60% nucleotide sequence identity (96.99–99.40% amino acid identity) with the corresponding sequence of six other BVQ isolates available in the GenBank. Iranian isolates displayed the highest nucleotide and amino acid sequence identities of 98.80-99.60% and 98.19-99.40%, respectively, with the French isolate FP71 (AJ810289). To our knowledge, this is the first molecular characterization of BVQ in Iran. This information can be used in plant breeding to obtain virus-resistant plants.

Keywords

Soilborne virus

Sugar beet

RT-PCR

Beet virus Q

20.1001.1.22519041.2021.10.3.12.0

Subjects

Plant Virology

Ali, A., Schneider, W.L., Sherman, D.J., Gray, S., Smith, D., Roosinck, M.J. 2006. Analysis of genetic bottlenecks during horizontal transmission of cucumber mosaic virus. Journal of Virology, 80: 8345–8350.

Barbarossa, L., Vetten, H. J., Kaufmann, A., Lesemann, D.-E., Koenig, R. 1992. Monoclonal antibodies to beet soil-borne virus. Annals of Applied Biology, 121: 143–150.

Borodynko, N., Rymelska, N., Hasiow-Jaroszewska, B., Pospieszny, H., 2009a. Molecular characterization of three soil-borne sugar beet-infecting viruses based on the coat protein gene. Journal of Plant Pathology, 91: 191-193.

Borodynko, N., Hasiów-Jaroszewska, B., Rymelska, N., Pospieszny, H. 2009b. Full length genome sequence of Polish isolate of Beet soil-borne virus confirms low level of genetic diversity. Acta Biochimica Polonica, 56: 729-731.

Crutzen, F., Mehrvar, M., Gilmer, D., Bragard, C. 2009. A full-length infectious clone of beet soil-borne virus indicates the dispensability of the RNA-2 for virus survival in planta and symptom expression on Chenopodium quinoa leaves. Journal of General Virology, 90: 3051-3056.

Erkan, E., Kutluk Yilmaz, N.D. 2017. Prevalence of Beet Virus Q in Sugar Beet Production Areas of Turkey. Journal of Turkish Phytopathology, 46: 53-60.

Farzadfar, S., Pourrahim, R., Golnaraghi, A.R., Ahoonmanesh, A. 2005. First Report of Beet virus Q on Sugar Beet in Iran. Plant Disease, 89(12): 1359.

Farzadfar, S., Pourrahim, R., Golnaraghi, A.R. Ahoonmanesh, A. 2007. Surveys of Beet necrotic yellow vein virus, Beet soil-borne virus, Beet virus Q and Polymyxa betae in sugar beet fields in Iran. Journal of Plant Pathology, 89: 277-281.

French, R., Stenger, D.C. 2003. Evolution of Wheat streak mosaic virus: dynamics of population growth within plants may explain limited variation. Annual Review of Phytopathology, 41: 199–214.

King, A.M.Q., Adams, M.J., Carstens, E.B., Lefkowitz, E.J. 2012. Virus taxonomy: Classification and nomenclature of viruses. Ninth report of the International Committee on Taxonomy of Viruses. San Diego: Elsevier/Academic Press. 1327 p.

Koenig, R., Pleij, C. W. A., Beier, C. & Commandeur, U. (1998). Genome properties of Beet virus Q, a new furo-like virus from sugarbeet, determined from unpurified virus. Journal of General Virology, 79: 2027–2036.

Kumar, S., Stecher, G., Li, M., Knyaz, C., Tamura, K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35: 1547–1549.

Lennefors, B.L., Savenkov, E.I., Mukasa, S.B., Valkonen, J.P. 2005. Sequence divergence of four soilborne sugarbeet-infecting viruses. Virus Genes, 31: 57-64.

Lesemann, D.-E., Koenig, R., Lindsten, K. Henry, C. 1989. Serotypes of beet soil-borne furovirus from FRG and Sweden. EPPO Bulletin, 19: 539–540.

Mehrvar, M. 2009. Diversity of soil-borne sugar beet viruses in Iran A comprehensive study of Beet necrotic yellow vein virus, Beet black scorch virus and other pomoviruses in Iran. PHD thesis, The University of Louvain, Belgium.

Meunier, A., Schmit, J.F., Stas, A., Kutluk, N. & Bragard, C. 2003. Multiplex reverse transcription-PCR for simultaneous detection of Beet necrotic yellow vein virus, Beet soilborne virus, and Beet virus Q and their vector Polymyxa betae KESKIN on sugar beet. Applied and Environmental Microbiology, 69: 2356-2360.

Muhire, B.M., Varsani, A., Martin, D.P. 2014. SDT: a virus classification tool based on
pairwise sequence alignment and identity calculation. PLoS ONE, 9(9): e108277.

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