Morphological and molecular identification of Fusarium spp. associated with carnation Dianthus caryophyllus in Mahallat, Iran

Shahbazi, Samira; Zaker Tavallaie, Fatemeh; Daroodi, Zoha

doi:10.48311/jcp.2021.1547

Morphological and molecular identification of Fusarium spp. associated with carnation Dianthus caryophyllus in Mahallat, Iran

https://doi.org/10.48311/jcp.2021.1547

Volume 10, Issue 3
September 2021

Pages 461-471

Document Type : Original Research

Authors

S. Shahbazi ¹

F. Zaker Tavallaie ²

Z. Daroodi ³

¹ Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute, Alborz, Iran.

² Department of Plant Production, Faculty of Agriculture, Higher Education Complex of Shirvan, Shirvan, Iran.

³ Department of Plant Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.

Abstract

Carnation is an ornamental plant of economic importance worldwide. Fusarium fungus is one of the critical infectious agents of Carnation in Mahallat city. Some species of Fusarium cause wilting and crown and root rot in carnation. In this study, 20 fungal isolates of Fusarium were isolated from diseased carnation plants and rhizosphere samples from Carnation greenhouses in Mahallat. The isolates were identified morphologically using Leslie and Summerell’s identification key. Amplification of the TEF-1α gene region was done using ef1 and ef2 primers. Amplified fragments were purified and sequenced, and the nucleotide sequences were introduced in the Fusarium ID database for molecular identification of isolates based on the TEF-1α gene. A phylogenetic tree was also drawn. The results showed that Fusarium isolates were categorized into five species: F14 was identified as Fusarium brachygibbosum morphologically and F. solani molecularly. F6 was morphologically and molecularly identified as F. solani, F10 identified as Fusarium culmorum. All crown and root isolates (F11, F12, F13, F15, F16, F17, F18, F9, and F20) were identified as F. oxysporum. The rhizosphere isolates of F1, F2, F3, F4, F5, F7, F8, and F9 were identified as Fusarium proliferatum. This study shows that the use of the TEF-1α gene for molecular identification of Fusarium isolates is a relatively accurate, fast, and straightforward method for detecting species and can be used in plant pathological studies. Identifying the pathogen is the first step to controlling the disease.

Keywords

Carnation

Fusarium

Molecular Identification

TEF-1α gene

20.1001.1.22519041.2021.10.3.7.5

Subjects

Mycology and Fungal Plant Diseases

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