Comparative study of virulence of Ophiognomonia leptostyla

Khelgatibana, Fatemeh; Javan-Nikkhah, Mohammad; Safaie, Naser; Fotouhifar, Khalil-Berdi; Vahdati, Kourosh; Ebrahimie, Esmaiel

doi:10.48311/jcp.2020.1480

Comparative study of virulence of Ophiognomonia leptostyla

https://doi.org/10.48311/jcp.2020.1480

Volume 9, Issue 2
June 2020

Pages 307-318

Document Type : Original Research

Authors

F. Khelgatibana ¹

M. Javan-Nikkhah ²

N. Safaie ³

K. Fotouhifar ¹

K. Vahdati ⁴

E. Ebrahimie ⁵

¹ Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

² 1. Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

³ Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

⁴ Department of Horticulture, College of Aburaihan, University of Tehran, Tehran, Iran.

⁵ Institute of Biotechnology, School of Agriculture, Shiraz University, Shiraz, Iran.

Abstract

Anthracnose disease caused by Ophiognomonia leptostyla, is the most important and widespread fungal disease on Juglans regia. Walnut disease symptomatic samples were collected from different provinces of Iran, during 2015–2016. Fungal isolates were identified based on ITS-rDNA sequence data. Variance analysis of colony growth rate (mm/day) and acervulus density on medium, was significant. Acervulus density on medium was strongly correlated with colony growth rate. The Max acervulus density was 60% and > 80% for Hamedan and Mazandaran isolates respectively. The virulence of six selected isolates was examined on cv. Chandler. Virulence indices including spot diameter, disease severity, spot area average and logistic infection rate except spot number index, could successfully detect significant differences among isolates. SA-SE1 isolate from Mazandaran showed significantly the most virulence indices: disease severity (%), spot area and logistic infection rate. For the other five isolates, four significant levels in all virulence indices were observed. In summary after this isolate, other isolates including TA-ZY21, LA-SY21, U94-SR1, HA-GH22 and MA-K1 were placed in the next steps of virulence ranking. There was insignificant correlation between colony growth rate and disease severity. However, the acervulus density and disease severity were significantly correlated implying the importance of acervular conidial inoculum in secondary disease cycle progress. Disease severity was strongly correlated with number of spots, spot diameter and logistic infection rate. Disease severity was also negatively correlated with Mid-time (time to progress 50%). Moreover, there was positive relationship between logistic infection rate and three traits: number of spots, spot diameter and spot area average. This study was the first of the disease virulence components on cv. Chandler in Iran.

Keywords

cv. Chandler

disease severity

Virulence

walnut anthracnose

20.1001.1.22519041.2020.9.2.12.1

Subjects

Mycology and Fungal Plant Diseases

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