Vector transmission of lime witches' broom Phytoplasma to Mexican lime seedlings under greenhouse condition

Hemmati, Chamran; Askari Seyahooei, Majeed; Nikooei, Mehrnoosh; Modarees Najafabadi, Seyed Saeid; Goodarzi, Azadeh; Amiri Mazraie, Mohsen; Faghihi, Mohammad Mehdi

doi:10.48311/jcp.2020.1472

Vector transmission of lime witches' broom Phytoplasma to Mexican lime seedlings under greenhouse condition

https://doi.org/10.48311/jcp.2020.1472

Volume 9, Issue 2
June 2020

Pages 209-215

Document Type : Brief Communication

Authors

C. Hemmati ¹

M. Askari Seyahooei ²

M. Nikooei ³

S. S. Modarees Najafabadi ²

A. Goodarzi ²

M. Amiri Mazraie ²

M. M. Faghihi ²

¹ Department of Agriculture, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran. Plant Protection Research Group, University of Hormozgan, Bandar Abbas, Iran.

² Plant Protection Research Department, Hormozgan Agricultural and Natural Resources Research and Education Center, Agricultural Research Education and Extension Organization (AREEO), Bandar Abbas, Iran.

³ Department of Agriculture, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran.

Abstract

Despite successful lime witches'-broom (LWB) phytoplasma transmission by Hishimonus phycitis to the lime trees and Bakraee seedlings, there is no published document regarding LWB transmission by H. phycitis to lime seedlings. To study the possibility of vector-based transmission to lime seedlings, the feral leafhoppers were collected in LWB-infected lime orchards and caged on one-year old Mexican lime seedlings. Six months after inoculation, 50% of inoculated seedlings showed typical symptoms of LWB and were strongly positive in PCR assays. To our knowledge, this is the first report of transmission of Ca. P. aurantifolia to Mexican lime seedlings by H. phycitis under greenhouse condition.

Keywords

Hishimonus phycitis

Mexican lime seedlings

16SrII phytoplasma

LWB disease

20.1001.1.22519041.2020.9.2.4.3

Subjects

Plant Disease Epidemiology

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