Reduction of aflatoxin production by exposing Aspergillus flavus to CO2

Mahbobinejhad, Zohre; Aminian, Heshmatolah; Ebrahimi, Leila; Vahdati, Kourosh

doi:10.48311/jcp.2019.1448

Reduction of aflatoxin production by exposing Aspergillus flavus to CO2

https://doi.org/10.48311/jcp.2019.1448

Volume 8, Issue 4
August 2019

Pages 441-448

Document Type : Original Research

Authors

Z. Mahbobinejhad ¹

H. Aminian ¹

L. Ebrahimi ¹

K. Vahdati ²

¹ Department of Entomology and Plant Pathology, College of Aburaihan, University of Tehran, Tehran, Iran.

² Department of Horticulture, College of Aburaihan, University of Tehran, Tehran, Iran.

Abstract

Aspergillus flavus is a saprophytic fungus contaminating different food and nut products by aflatoxin which is a major problem worldwide. Modified atmosphere packaging (MAP) could be an effective method for control of saprophytic fungi and their toxins and secondary metabolites production. This study gives the consequences of fungal isolates growing under MAP condition on potato dextrose agar (PDA). Two isolates of A. flavus (A42 and CHAO50) were packed under 100% CO₂, 100% O₂and vacuum conditions. The mycelial growth of fungal isolates on PDA was controlled up to 80% with CO₂ treatment. Under the vacuum condition, mycelial growth of the isolates was inhibited up to 30%, while 100% oxygen had no inhibition on mycelial growth. Examination of isolates A42 and CHAO50 under UV light showed that both isolates produced yellow pigments on aflatoxin producing ability (APA) medium after 10 days. The levels of aflatoxin B₁, B₂, G₁, G₂ and total aflatoxin were analyzed chromatographically. The results revealed that the highest concentration of total aflatoxin was produced by fungal isolates grown in an atmosphere of 100% O₂, while the level of aflatoxins was significantly reduced in 100% CO₂.

Keywords

Keywords: HPLC

Modified atmosphere

Aflatoxin

vacuum condition

20.1001.1.22519041.2019.8.4.7.3

Subjects

Plant Disease Management

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