Molecular characterization of a 16SrIX phytoplasma associated with Convolvulus glomeratus witches' broom and with an insect vector in Iran

Nikooei, Mehrnoosh; Hemmati, Chamran

doi:10.48311/jcp.2018.1402

Molecular characterization of a 16SrIX phytoplasma associated with Convolvulus glomeratus witches' broom and with an insect vector in Iran

https://doi.org/10.48311/jcp.2018.1402

Volume 7, Issue 4
December 2018

Pages 387-393

Document Type : Brief Communication

Authors

M. Nikooei ¹

C. Hemmati ²

¹ Department of Agriculture, Minab Higher Education Center, University of Hormozgan, Bandar Abbas, Iran.

² Plant Protection Research Group, University of Hormozgan, Bandar Abbas, Iran.

Abstract

During a survey in 2016, Convolvulus glomeratus showing symptoms resembling those caused by phytoplasmas were observed in Bandar Abbas, Hormozgan province, Iran. These plants were examined for phytoplasma presence by nested-PCR assays using universal primer pair P1/P7 followed by R16F2n/R16R2. All the infected plants indicated positive results using universal primer pairs of P1/P7 followed by R16F2n/R16R2. Results of phylogenetic and virtual RFLP analyses of the 16S rRNA gene sequences revealed that the phytoplasma associated with Convolvulus glomeratus witches' broom (CgWB) was a strain of 'Candidatus Phytoplasma phoenicium'. The phytoplasma was successfully transmitted to healthy plants by leafhopper, Orosius albicinctus (Distant) which account as a vector of this phytoplasma. This is the first report on the presence of ‘Candidatus Phytoplasma phoenicium’ (phytoplasma group 16SrIX-J) in C. glomeratus and its insect vector in Iran.

Keywords

16SrIX

Convolvulus glomeratus

Orosius albicinctus

Bandar Abbas

witches' broom

20.1001.1.22519041.2018.7.4.6.5

Subjects

Prokaryotic Plant Diseases

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