Journal of Crop Protection

New reports on dixenic associations between the symbionts of entomopathogenic nematodes, Photorhabdus and Xenorhabdus, and non-symbiotic bacteria

https://doi.org/10.48311/jcp.2017.1363
Volume 6, Issue 4
December 2017
Pages 497-511
Journal of Crop Protection

Authors

N. Eivazian Kary 1
D. Mohammadi 1
R. Girling 2

1 Plant Protection Department, Faculty of Agriculture, Azarbaijan Shahid Madani University, Tabriz, Iran.

2 School of Agriculture, Policy and Development, Centre for Agri-Environmental Research, University of Reading, Reading, UK.

Abstract
By conducting three different methods, we report on the isolation of five novel strains of non-symbiotic bacteria from crushed infective juveniles (IJs) of four species of entomopathogenic nematodes (EPN) including Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema feltiae, and Steinernema glaseri and five bacterial species from hemolymph of insect larvae infected with EPNs. Samples of hemolymph of infected Galleria mellonella L. larvae by EPNs and crushed surface sterilized IJs were bulk streaked onto both MacConkey and NBTA agar. To further ensure diagnoses, extracted DNA from IJs bulk was subjected to PCR by 16S-rRNA bacterial universal primers. Bacteria were identified using biochemical and phylogenetic analysis. Based on 16S-rRNA gene sequence, maximum parsimony, maximum likelihood and neighbour joining phylogenetic analyses were conducted, as well as comparisons of predicted RNA secondary structures. Four species of bacteria were identified including: Stenotrophomonas maltophilia strain IR11 from S. feltiae; S. pavanii strain IR20 from S. glaseri; Acinetobacter junii strain IR8 from S. carpocapsae; and Alcaligenes faecalis strains IR1 & IR15 from S. feltiae and H. bacteriophora respectively as non-symbiotic bacteria from IJs and five species probably originated from G. mellonella intestine including Citrobacter gillenii isolate S3, Enterobacter asburiae isolate S4, Klebsiella oxytoca isolate S5, Morganella morganii isolate S6 and Serratia marcescens isolate S6.

Keywords

Stenotrophomonas maltophilia
S. pavanii
Acinetobacter junii
Alcaligenes faecalis
Citrobacter gillenii
Enterobacter asburiae Klebsiella oxytoca
Morganella morganii
Serratia marcescens
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