Bacterial pathogens associated with citrus huanglongbing-like symptoms in southern Iran

Saberi, Esmaeil; Alavi, Seyed Mehdi; Safaie, Naser; Moslemkhany, Cobra; Azadvar, Mehdi

doi:10.48311/jcp.2017.1329

Bacterial pathogens associated with citrus huanglongbing-like symptoms in southern Iran

10.48311/jcp.2017.1329

Volume 6, Issue 1
March 2017

Pages 99-113

Authors

E. Saberi ¹

S. M. Alavi ²

N. Safaie ¹

C. Moslemkhany ³

M. Azadvar ³

¹ Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

² Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.

³ Plant Protection Research Department, South Kerman Agricultural and Natural Resources Research Centre, AREEO, Jiroft, Iran.

Abstract

Huanglongbing (HLB) also known as citrus greening, is a destructive disease of citrus and now, is considered as a new emergence and spread out threat to the Middle East and North Africa (MENA) citrus production. In a survey conducted in southern Iran in 2013-2014, 77 citrus samples exhibiting symptoms of HLB were collected. Single-step and nested polymerase chain reactions (PCR) were employed to determine the presence of the phloem-limited bacterial pathogens ‘Candidatus Liberibacter asiaticus’(CLas), phytoplasma and Spiroplasma citri. Both CLas and phytoplasma were detected in HLB-affected citrus trees as co-infection (7.79%) and single infection (10.38% for phytoplasma and 42.85% for CLas). According to the proposed 16S rDNA-based phytoplasma classification scheme, the HLB-associated phytoplasma from this study was a member of peanut witches’broom (16SrII) phytoplasma group. This is the first report of association of a phytoplasma with HLB in sweet lime in the world and first record of association of CLas with sour orange (Citrus aurantium L.) and sweet lime in Iran.

Keywords

'Candidatus Liberibacter asiaticus'

Citrus greening

Phytoplasma

Citrus

Iran

20.1001.1.22519041.2017.6.1.14.0

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