The effect of environmental factors on surfactin production of Bacillus subtilis

Nazari, Fahimeh; Safaie, Naser; Soltani, Bahram Mohammad; Shams-Bakhsh, Masoud; Sharifi, Mohsen

doi:10.48311/jcp.2017.1328

The effect of environmental factors on surfactin production of Bacillus subtilis

10.48311/jcp.2017.1328

Volume 6, Issue 1
March 2017

Pages 89-97

Authors

F. Nazari ¹

N. Safaie ¹

B. M. Soltani ²

M. Shams-Bakhsh ¹

M. Sharifi ³

¹ Department of Plant Pathology, Tarbiat Modares University, Tehran, Iran.

² Department of Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

³ Department of Plant Sciences, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Abstract

Surfactin is one of the most efficient biosurfactants excreted by Bacillus subtilis which displays the highest potential as induced systemic resistance elicitor among all metabolites produced by B. subtilis. Environmental factors have considerable effect on surfactin production. In this study surfactin production of two Bacillus subtilis strains were analyzed using high performance liquid chromatography (HPLC). C14 and C15 surfactins were detected in the ethanol extract from acid-precipitated supernatant. HPLC analyses of different media including Nutrient Broth (NB) medium, NB plus 40g/l glucose, NB plus 10% soil extract and NB plus 10% plant extract medium, clearly showed that these bacteria produced different amounts of surfactins C14 and C15 in these media. Surfactin production in NB/plant medium was relatively the highest in quantity. Microelements analysis of media containing plant and soil extract with atomic absorption spectrometry showed high amounts of Fe, Mn and Zn in medium containing plant extract compared with that of soil extract. Since these elements play an important role in surfactin production, high amounts of Fe, Mn and Zn in NB/plant extract medium compared to the NB/soil extract medium could be the possible reason for relatively higher amounts of surfactins C14 and C15 produced in NB/plant medium. So adding these important elements to soil may boost biocontrol effect of B. subtilis against plant pathogens.

Keywords

Surfactin

HPLC

Bacillus spp

Biocontrol

20.1001.1.22519041.2017.6.1.10.6

Akpa, E., Jacques, P., Wathelet, B., Paquot, M., Fuchs, R., Budzikiewicz, H. and Thonart, P. 2001. Inﬂuence of culture conditions on lipopeptide production by Bacillus subtilis. Applied Biochemistry and Biotechnology, 91: 551-561.

Araújo, W. L. J., Marcon, W., Maccheroni, J. D. Jr., van Elsas, J. W. L. and Azevedo J. L. 2002. Diversity of endophytic bacterial populations and their interaction with Xylella fatidiosa in citrus plants. Applied and Environmental Microbiology, 68: 4906-4914.

Arima, K., Kakinuma, A. and Tamura, G. 1968. Surfactin, a crystalline peptide lipid surfactant produced by Bacillus subtilis: isolation, characterization and its inhibition of fibrin clot formation. Biochemical and Biophysical Research Communications, 31: 488-494.

Asad, A., Ikramullah, M., Asif, S., Ahmad, A., Jamal, A. and Jaffri, A. 2010. Evaluation of lipopeptide (surfactin) production by Bacillus subtilis. Biomedical, 26: 34-3.

Bais, H., Ray, F. P. and Vivanco, J. M. 2004. Biocontrol of Bacillus subtilis against Infection of Arabidopsis Roots by Pseudomonas syringae is facilitated by Biofilm Formation and Surfactin Production. Plant Physiology, 134: 307-319.

Baumgart, F., Kluge, B., Ullrich, C., Vater, J. and Ziessow, D. 1991. Identification of amino acid substitutions in the lipopeptide surfactin using 2D NMR spectroscopy. Biochemical and Biophysical Research Communications, 177: 998-1005.

Chayabutra, C., Wu, J. and Ju, L. K. 2001. Rhamnolipid production by Pseudomonas aeruginosa under denitrification: Effects of limiting nutrients and carbon substrates. Biotechnology and Bioengineering, 72 (1): 25-33.

Cooper, D. G., Macdonald, T. C. R., Duff, S. J. B. and Kosaric, N. 1981. Enhanced production surfactin from Bacillus subtilis by continuous product removal and metal cation additions. Applied and Environmental Microbiology, 42 (3): 408-412.

Eeman, A., Francius, G., Dufrene, Y.F., Nott, K., Paquot, A. and Deleu, A. 2009. Effect of cholesterol and fatty acids on the molecular interactions of fengycin with Stratum corneum mimicking lipid monolayers. Langmuir, 25: 3029-3039.

Guerra-Santos, L. H., Kappeli, O. and Fiechter, A. 1986. Dependence of Pseudomonas aeruginosa continous culture biosurfactant production on nutritional and environmental factors. Applied Microbiology and Biotechnology, 24 (6): 443-448.

Henry, G., Deleu, M., Jourdan, E., Thonart, P. h. and Ongena, M. 2011. The bacterial lipopeptide surfactin targets the lipid fraction of the plant plasma membrane to trigger immune-related defense responses. Cellular Microbiology, 13: 1824-1837.

Jacques, P., Hbid, C., Destain, J., Razaﬁndralambo, H., Paquot, M., De Pauw, E. and Thonart, P. 1999. Optimization of biosurfactant lipopeptide production from Bacillus subtilis S499 by Plackett-Burman design. Applied Biochemistry and Biotechnology, 77: 223-233.

Jourdan, E., Henry, G., Duby, F., Dommes, J., Barthelemy, J. P., Thonart, P. and Ongena, M. 2009. Insights into the defense-related events occurring in plant cells following perception of surfactin-type lipopeptide from Bacillus subtilis. Molecular Plant-Microbe Interactions, 22: 456-468.

Kim, K., Jung, S., Lee, S. Y., Jung, D. K., Park, J. K., Kim, D. K. and Lee, C. H. 1998. Suppression of inflammatory responses by surfactin, a selective inhibitor of platelet cytosolic phospolipase A2. Biochemical Pharmacology, 55 (7): 975-985.

Lang, S., Wullbrandt, D. and Rhamnose R. 1999. Lipids-biosynthesis, microbial production and application potential. Applied Microbiology and Biotechnology, 51 (1): 22-32.

Leila Santiago-Flores M. 1977. A Manual on Some Philippine Medicinal Plants (Preparation of drug materials). U. P. Botanical Society, 20: 78-82.

Lochhead, A. G. and Burton, M. 0. 1957. Qualitative studies of soil microrganisms. XIV. Specific vitamin requirements of the predominant bacterial flora. Canadian Journal of Microbiology, 3: 35-39.

Maget-dana, R. and Peypoux, F. 1994. Iturins, a specialclass for pore forming lipopeptides-biological and physicochemical properties. Toxicology, 87: 151-174.

Manouchehri, N., Besancon, S. and Bermond, A. 2006. Major and Trace Metal Extraction from Soil by EDTA: Equilibrium and Kinetic Studies. Analytica Chimica Acta, 559 (1): 105-112.

Moralejo, M. P. and Acebal, S. G. 2014. The Transfer of Cu, Zn, Mn and Fe between Soils and Allium Plants (Garlic and Onion), and Tomato in the Southwest of the Buenos Aires Province, Argentina. American Journal of Plant Sciences, 5: 480-487.

Nitschke, M., Costa, S. G. V. A. O. and Contiero, J. 2005. Rhamnolipid surfactants: An update on the general aspects of these remarkable biomolecules. Biotechnology Progress, 21: 1593-1600.

Oka, K., Hirano, T., Homma, M., Ishii, H., Murakami, K., Mogami, S., Motizuki, A., Morita, H., Takeya, K. and Itokawa, H. 1993. Satisfactory separation and MS-MS spectrometry of six surfactins isolated from Bacillus subtilis natto. Chemical and Pharmaceutical Bulletin, 41: 1000-1002.

Ongena, M. and Jacques, P. 2008. Bacillus lipopeptides: versatile weapons for plant disease biocontrol. Trends in Microbiology, 16: 115-125.

Preecha, C., Sadowsky, M. J. and Prathuangwong, S. 2010. Lipopeptide surfactin produced by Bacillus amyloliquefaciens KPS46 is required for biocontrol efficacy against Xanthomonas axonopodis pv. Glycines. Kasetsart Journal (Natural Science), 44: 84-99.

Raaijmakers, J. M., de Bruijn, I., Nybroe, O. and Ongena, M. 2010. Natural functions of lipopeptides from Bacillus and Pseudomonas: more than surfactants and antibiotics. FEMS Microbiology Reviews 34: 1037-1062.

Singh, P. and Cameotra S. S. 2004. Potential applications of microbial surfactants in biomedical sciences. Trends in Biotechnology, 22: 142-146.

Wei, Q. F., Mather, R. R. and Fotheringham, A. F. 2005. Oil removal from used sorbents using abiosurfactant. Bioresource Technology, 96:331-334.

Wei, Y. H., Wang, L. F. and Chang, J. S. 2004. Optimizing iron supplement strategies for enhanced surfactin production with Bacillus subtilis. Biotechnology Progress, 20: 979-983.

Yeh, M. S., Wei, Y. H. and Chang, J. S. 2005. Enhanced production of surfactin from Bacillus subtilis by addition of solid carriers. Biotechnology Progress, 21: 1329-1334.

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