Effects of some micronutrients and macronutrients on the root-knot nematode, Meloidogyne incognita, in greenhouse cucumber (Cucumis sativus cv. Negin)

10.48311/jcp.2016.1307
Volume 5, Issue 4
December 2016
Pages 507-517

Authors

1 Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz, Iran.

2 Department of Plant Protection, College of Agriculture, Yasouj University, Yasouj, Iran.

Abstract
The effects of some micronutrients (iron, zinc and silicon) and macronutrients (nitrogen, phosphorus and potassium) were evaluated on the root-knot nematode, Meloidogyne incognita and plant growth parameters of cucumber (Cucumis sativus cv. Negin) in two independent trials. Each of iron, zinc and silicon micronutrients was used at 5 mg/kg of soil, as iron sequestrene (Fe-EDDHA), zinc sulfate (ZnSO4) and sodium siliconate (Na2O3Si), respectively. Furthermore, nitrogen at 60, 120 and 180 mg/kg, phosphorus at 25, 50 and 75 mg/kg and potassium at 12.5, 25 and 37.5 mg/kg of soil were used as urea, triple superphosphate and potassium sulfate, respectively. At four-leaf stage seedlings, 8000 nematode eggs and juveniles (2 egg and juveniles/gr soil) were added around the roots. After 60-days, data analysis indicated silicon + iron, significantly reduced the number of galls/g of root by 55 and 42% compared to control, in the two experiments, respectively, but none of these treatments resulted in significant positive effects on the growth or yield of the studied cucumber cultivar. When macronutrients were evaluated, results showed that N120P25K25 (120 mg/kg of Nitrogen, 25 mg/kg of phosphorus and 25 mg/kg of potassium) and N120P50K25 (120 mg/kg of nitrogen, 50 mg/kg of phosphorus and 25 mg/kg of potassium) significantly reduced the number of galls by 96 and 81% (experiment 3) and 79 and 70% (experiment 4) when compared with control, respectively. These both treatments also improved cucumber growth parameters such as shoot dry and fresh weights, root fresh weight and fruit yield.

Keywords

Abolusoro, S. A., Abolusoro, P. F., Izuogu, N. B. and Mathew, F. O. 2013. Effects of organic and inorganic manures on the growth attributes of root-knot nematode (Meloidogyne incognita) infected Ethiopian eggplant (Solanum aethiopicum).World Journal of Agricultural Research, 1: 104-107.
Akhtar, A., Hisamuddin, A. and Sharf, R. 2013. Study on Black Gram (Vigna mungo L.) infected with Meloidogyne incognita under the influence of Pseudomonas fluorescens, Bacillus subtilis and urea. Journal of Plant Pathology and Microbiology, 4 (9): 202. doi:10.4172/2157-7471.1000202.
Asano, S. and Moura, R. M. 1995. Efeitos dos macro e micronutrients na severidade da meloidoginose da cana-de-acúcar. Nematologia Brasiliera, 19: 15-20.
Back, M. A., Haydock, P. P. J. and Jenkinson, P. 2002. Disease complexes involving plant parasitic nematodes and soil borne pathogens. Plant Pathology, 51: 683-697.
Badra, T. and Khattab, M. M. 1980. The effect of nitrogen fertilizers on the growth of olive and in relation to infestation of Rotylenchulus reniformis. Nematologia Mediterranea, 8: 67-72.
Charegani, H., Karegar, A. and Hamzehzarghani, H. 2010. Effect of chemical fertilizers on root-knot nematode (Meloidogyne incognita) in greenhouse cucumber cultivation. Iranian Journal of Plant Pathology, 46: 263-274.
Crow, W. T., Guertal, E. A. and Rodriguez-Kabana, R. 1996. Responses of Meloidogyne arenaria to green manures and supplemental urea in glasshouse culture. Supplement to Journal of Nematology, 28: 648-654.
Hartman, K. M., and Sasser, J. N. 1985. Identification of Meloidogyne species on the basis of differential host test and perineal-pattern morphology. In: Barker, K. R., Carter, C. C. and Sasser, J. N. (Eds). An Advanced Treatise on Meloidogyne, Vol. 2, Methodology. North Carolina State University Graphics, Raleigh, USA. pp: 69-77.
Hunt, D. J. and Handoo, Z. A. 2009. Taxonomy, identification and principal species. In: Perry R. N., Moens, M. and Starr, J. L. (Eds). Root-Knot Nematodes. CABI Publishing. pp: 55-97.
Hussey, R. S. and Barker, K. 1973. A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Plant Disease Reporter, 57: 1025-1028.
Hussey, R. S. and Janssen, G. J. W. 2002. Root-knot nematode: Meloidogyne species. In: Starr, J. L., Cook, R. and Bridge, J. (Eds.). Plant Resistance to Parasitic Nematodes. CAB International, UK. pp: 69-77.
Hussey, R. S. and Roncadori, R. N. 1982. Vesicular arbuscular mycorrhizae may limit nematode activity and improve plant growth. Plant Disease, 66: 9-14.
Irshad, M., Asmat, S. and Rose, R. 2011. Interaction between Pseudomonas fluorescens and Meloidogyne incognita on tomato plant as influenced by the different levels of phosphorus. Archives of Phytopathology and Plant Protection, 44: 993-1000.
Ismail, A. E., Abd-el-Migeed, M. M., Azza, R. A. and Awaad, M. S. 2011. Meloidogyne incognita suppression and changes of grapevine yield properties determined by waste residues from jojoba, black seed oil extraction and slow release of nitrogen fertilizer. Pakistan Journal of Nematology, 29: 187-205.
Mahmoud, E., Abd El-Kader, N., Robin, P., Akkal-Corfini, N. and Abd El-Rahman, L. 2009. Effects of different organic and inorganic fertilizers on cucumber yield and some soil properties. World Journal of Agricultural Sciences, 5: 408-414.
Marschner, H. 1997. Mineral Nutrition of Higher Plants. Academic Press, London. 889 pp.
Nasr Esfahani, M. and Ahmadi, A. 2005. Effects of organic amendments and chemical fertilizers on Meloidogyne javanica in cucumber. Iranian Journal of Plant Pathology, 41: 1-17.
Noweer, E. M. A. and Hasabo, S. A. 2005. Effect of different management practices for controlling root-knot nematode Meloidogyne incognita on squash. Egyptian Journal of Phytopathology, 33: 73-81.
Pant, V., Hakim, S. and Saxena, S. K. 1983. Effect of different levels of N, P, K on the growth of tomato Marglobe and on the morphometrics of root-knot nematode Meloidogyne incognita. Indian Journal of Nematology, 13: 110-113.
Parveen, G., Ehteshamul-Haque, Sultana, V., Ara, J. and Athar, M. 2008. Suppression of root pathogens of tomato by rhizobia, Pseudomonas aeruginosa, and mineral fertilizers. International Journal of Vegetable Science, 14: 205.
Persson, L. and Olsson, S. 2000. Abiotic characteristic of soils suppressive to Aphanomyces root rot. Soil Biology and Biochemistry, 32: 1141-1150.
Rodriguez-Kábana, R. 1986. Organic and inorganic nitrogen amendments to soil as nematode suppressants. Journal of Nematology, 18: 129-135.
Rodriguez-Kábana, R., King, P. S. and Pope, M. H. 1981. Combinations of anhydrous ammonia and ethylene dibromide for control of nematodes parasitic on soybeans. Nematropica, 11: 27-41.
Santana-Gomez, S. M., Dias-Arieira, C. R., Roldi, M., Dadazio, T. S., Marini, P. M. and Barizao, D. A. O. 2013. Mineral nutrition in the control of nematodes. African Journal of Agricultural Research, 8: 2413-2420.
 
 
 
 
 
 
 
Sasser, J. N. and Taylor, A. L. 1978. Biology, Identification, and Control of Root-Knot Nematodes (Meloidogyne species). Department of Plant Pathology, North Carolina State University and the United States Agency for International Development,111 pp.
Siddiqui, Z. A., Iqbal, A. and Mahmood, I. 2001. Effects of Pseudomonas fluorescens and fertilizers on the reproduction of Meloidogyne incognita and growth of tomato. Applied Soil Ecology, 16: 179-185.
Sikora, R. A. and Frenández, E. 2005. Nematode parasites of vegetables. In: Luc. M., Sikora, R. A. and Bridge, J. (Eds.). Plant Parasitic Nematodes in Subtropical and Tropical Agriculture, 2nd edition. CABI Publishing, Wallingford, UK. pp: 319-392.
Viaene, N., Coyne, D. L. and Davies, K. G. 2013. Biological and cultural management. In: Perry R. N. and Moens, M. (Eds.). Plant Nematology, 2nd edition. CABI Wallingford, UK. pp: 383-410.
Wang, E. L. H. and Bergeson, G. B. 1974. Biochemical changes in root exudate and xylem sap of tomato plants infected with Meloidogyne incognita. Journal of Nematology, 6: 194-202.
Whitehead, A. G. and Hemming, J. R. 1965. A comparison of some quantitative methods of extracting vermiform nematodes from soil. Annals of Applied Biology, 55: 25-38.
Youssef, M. M. A., Mohammed, M. M. M. and Korayam, A. M. 2012. Effect of organic and inorganic fertilizers on Meloidogyne incognita infesting sugar beet. Pakistan Journal of Nematology, 30: 143-149.