Xanthogalerucella luteola (Col.: Chrysomelidae) α-amylase affected by seed proteinaceous extract from datura, wild oat and amaranth seeds

10.48311/jcp.2016.1290
Volume 5, Issue 2
June 2016
Pages 157-167

Authors

Plant Protection Department, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Abstract
The elm leaf beetle, Xanthogalerucella luteola (Muller) (Col.: Chrysomelidae) is a serious pest of elm trees and it has been distributed all over the world. The current study was undertaken to investigate the inhibitory effects of protein extracts of three weed seeds including datura Datura stramonium L., amaranth Amaranthus retroflexus L. and wild oat Avena fatua L. against X. luteola α-amylase using spectrophotometric assay as well as in gel assays. The effects of five concentrations of each seed proteinaceous extracts were tested on α-amylase activity of the larval gut. The results showed a dose dependent manner in inhibition of the insect enzyme. At the highest concentration of protein extracts (12 μg protein) of all three seed extracts including amaranth, wild oat and datura, the inhibition was 71, 79 and 31%, respectively. Whilst, at low concentration (0.75 μg protein), the inhibition observed was 15, 36 and 5%, respectively. Thus, the greatest inhibition percentage was obtained when proteinaceous extract of wild oat seed was used. These results were confirmed when in gel assays were performed. All three seed proteinaceous extracts had an optimum pH inhibition of 6.0. Thus, it is concluded that wild oat seed proteins are potentially good for detailed investigation in order to get a clear picture of its active compound/s and its structure-function relationship.

Keywords

Baker, J. E. 1987. Purification of isoamylases from the rice weevil, Sitophilus oryzae L. by HPLC and their interaction with partially purified amylase inhibitor from wheat. Insect Biochemistry and Molecular Biology, 17:37-44.
Bandani, A. R., Kazzazi, M. and Mehrabadi, M. 2009. Purification and characterization of midgut α-amylases of Eurygaster integriceps. Entomological Science, 12: 25-32.
Bernfeld, P. 1955. Amylase, α and β. Methods in Enzymolology, 1: 149-151.
Bradford, M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72: 248-254.
Dastranj, M., Bandani, A. R., and Mehrabadi, M. 2013. Age-specific digestion of Tenebrio molitor (Coleoptare: Tenebrionidae) and inhibition of proteolytic and amylolytic activity by plant proteinaceous seed extracts. Journal of Asia-Pacific Entomolog,16: 309-315.
Down, R. E., Fitches, EC., Wiles, D. P., Corti, P., Bell, H. A., Gatehouse, J. A. and Edwards, J. P. 2006. Insecticidal spider venom toxin fused to snowdrop lectin is toxic to the peach-potato aphid, Myzus persicae (Hemiptera: Aphididae) and the rice brown planthopper, Nilaparvata lugens (Hemiptera: Delphacidae). Pest Management Science, 62: 77-85.
Dreistadt, S., Dahlsten, D. and Lawson, A. 2001. Elm Leaf Beetle. Publication 7403. UC Statewide IPM Program, University of California, Davis, CA.
Fitches, E., Edwards, M. G., Mee, C., Grishin, E., Gatehouse, A. M. R., Edwards, J. P. and Gatehouse, J. A. 2004. Fusion proteins containing insect-specific toxins as pest control agents: snowdrop lectin delivers fused insecticidal spider venom toxin to insect haemolymph following oral ingestion. Journal of Insect Physiology, 50: 61-71.
Fitches, E., Wiles, D., Douglas, A. E., Hinchliffe, G., Audsley, N. and Gatehouse, J. A. 2008. The insecticidal activity of recombinant garlic lectins towards aphids. Insect Biochemistry and Molecular Biology, 38: 905-915.
Gatehouse, A. M., Norton, E., Davison, G. M., Babbé, S. M., Newell, C. A. and Gatehouse, J. A. 1999. Digestive proteolytic activity in larvae of tomato moth, Lacanobia oleracea; effects of plant protease inhibitors in vitro and in vivo. Journal of Insect Physiology, 45: 545-558.
Giri, A. P. and Kachole, M. S. 1996. Detection of electrophoretically separated amylase inhibitors in starch polyacrylamide geles. Journal of Chromatography, 752: 1538-22730.
Hao, X., Li, J., Shi, Q., Zhang, J., He, X. and Ma, H. 2009. Characterization of a novel legumin. Biochemistry, 73: 1200-1202.
Harrison, R. L. and Bonning, B. C. 2010. Proteases as insecticidal agents. Toxin 2: 935-953.
Hosseinkhani, S. and Nemat-Gorgani, M. 2003. Partial unfolding of carbonic anhydrase provides a method for its immobilization on hydrophobic adsorbents and protects it against irreversible thermoinactivation. Enzyme and Microbial Technology, 33: 179-184.
Huerta, A., Chiffelle, I., Puga, K., Azúa, F. and Araya, J. E. 2010. Toxicity and repellence of aqueous and ethanolic extracts from Schinus molle on elm leaf beetle Xanthogaleruca luteola. Crop Protection, 29: 1118-1123.
Isman, M. B. 2000. Plant essential oils for pest and disease management. Crop Protection, 19: 603-608.
Isman, M. B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annual Review of Entomology, 51: 45-66.
Kazzazi, M., Bandani, A. R. and Hosseinkhani, S. 2005. Biochemical characterization of α –amylase of the Sunn pest, Eurygaster integriceps. Entomological Sciences, 8: 371-377.
Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of bacteriophage T4. Nature, 227: 680-685.
Lajolo, F. M. and Finardi-Filho, F. 1985. Partial characterization of the amylase inhibitor of black beans (Phaseolus vulgaris), variety Rico 23. Journal of Agricultural and Food Chemistry, 33: 132-138.
Majidiani, S., Pour Abad, R. F. and Bandani, A. 2014. Effect of protein extracts of three bean varieties against gut α-amylase activity of Helicoverpa armigera (Hübner) (Lep.: Noctuidae). Archives of Phytopathology and Plant Protection, 47: 259-267.
Marshall, J. J. and Lauda, C. M. 1975. Purification and properties of phaseolamin, an inhibitor of α-amylase, from the kidney bean Phaseolus vulgaris. Journal of Biological Chemistry, 250: 8030-8037.
Mehrabadi, M., Bandani, A. R. and Kwon, O. 2011. Biochemical characterization of digestive α-β-glucosidase and α-β-glucosidase from labial glands and midgut of wheat bug Eurygaster Maura (Hemiptera: Scutelleridae). Entomological Research, 41: 81-87.
Mehrabadi, M., Bandani, A. R., Mehrabadi, R. and Alizadeh, H. 2012. Inhibitory activity of proteinaceous α- amylase inhibitors from triticale seeds against Eurygaster integriceps salivary α-amylases: interaction of the inhibitors and the insect digestive enzymes. Pesticide Biochemistry and Physiology, 102: 220-228.
Morton, R. L., Schroeder, H. E., Bateman, K. S., Chrispeels, M. J., Armstrong, E. and Higgins, T. J. V. 2000. Bean alpha-amylase inhibitor 1 in transgenic peas (Pisum sativum) provides complete protection from pea weevil (Bruchus pisorum) under field conditions. Proceedings of the National Academy of Sciences, 97: 3820-3825.
Muñoz, C., Pérez, V., Cobos, P., Hernández, R. and Sánchez, G. 2003. Sanidad Forestal. Mundi-Prensa, Madrid.
Powers, J. R. and Whitaker, J. R. 1977. Effect of several experimental parameters on combination of red kidney bean (Phaseolus vulgaris) α-amylase inhibitor with porcine pancreatic α-amylase. Journal of Food Biochemistry, 1: 239-260.
Rahimi, V. and Bandani, A. R. 2014. Comparison of the effects of cereal and legume proteinaceous seed extracts on α-amylase activity and development of the Sunn pest. Journal of Asia-Pacific Entomology, 17: 7-11.
Regnault-Roger, C., Philogène, B. J. R. and Vincent, C. 2002. Biopesticides d’origine végétale [Biopesticides of plant origin]. Techniques and Documents, Paris: Lavoisier, 337 pp..
Romanyk, M. and Cadahia, D. 2002. Plagas de insectos en las masas forestales. Mundi-Prensa, Madrid.
Saadati, F., Bandani, A. R. and Moslemi, A. 2011. Effect of plant seeds protein extract on the Sunn pest, Eurygaster integriceps Puton, growth and development and its gut serine protease activity. African Journal of Biotechnology, 10: 11502-11510.
Sharifi, M., Ghadamyari, M., Moghadam, M. M. and Saiidi, F. 2011. Biochemical characterization of digestive carbohydrases from Xanthogaleruca luteola and inhibition of its α-amylase by inhibitors extracted from the common bean. Archives of Biological Sciences, 63: 705-716.
Sharma, H. and Ortiz, R. 2000. Transgenics, pest management, and the environment. Current Science, 79: 421-437.
Sivakumar, S., Mohan, M., Franco, O. L. and Thayumanavan, B. 2006. Inhibition of insect pestα amylases by little and finger millet inhibitors. Pesticide Biochemistry and Physiology, 85: 155-160.
Tatli, I., Bandani, A. R. and Moslemi, A. 2013. The elm leaf beetle α-amylase and its activity relationship with insect feeding. Archives of Phytopathology and Plant Protection, 46: 917-926.
Valencia, A., Bustillo, A. E., Ossa, G. E and Chrispeels, M. J. 2000. α-Amylases of the coffee berry borer (Hypothenemus hampei) and their inhibition by two plant amylase inhibitors. Insect Biochemistry and Molecular Biology 30: 207-213.