Response of tomato cultivars to agroinfection with Beet curly top Iran virus

Khoshnazar, Farnaz; Eini, Omid

doi:10.48311/jcp.2016.1289

Response of tomato cultivars to agroinfection with Beet curly top Iran virus

10.48311/jcp.2016.1289

Volume 5, Issue 3
September 2016

Pages 473-482

Authors

F. Khoshnazar

O. Eini

Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.

Abstract

Beet curly top Iran virus (BCTIV) is a member of the genus Becurtovirus (family Geminiviridae) which constrain host crop production in various geographical regions in Iran. This virus infects several crops such as sugar beet Beta vulgaris and tomato Solanum lycopersicum. BCTIV infected tomato shows leaf curling, leaf distortion and stunting. In this study, we investigated the response of various tomato cultivars to BCTIV infection using an infectious clone of BCTIV under glasshouse condition at the University of Zanjan in 2013-2014. Based on a completely randomized design experiment twelve tomato cultivars were tested for their reaction to BCTIV infection. The replication of virus and symptom production was recorded and analyzed. Based on the obtained coefficient of infection and disease severity index, none of the tested cultivars was found resistance to the virus infection. However, one of the tested cultivars, Super Chief, showed no leaf curling symptom and the virus replicated at a significantly lower level in this cultivar as compared to a susceptible cultivar, Alindi 811, using quantitative PCR. Tomato cultivars including Grosse Lisse and Early Eurbana were grouped as susceptible while the other cultivars including Super Star were moderately susceptible to the virus infection. Therefore, growing this cultivar shows promise for an increase of yields from tomato plants prone to BCTIV infection after complementary field experiments. The screening of more cultivars or wild-type tomatoes for the identification of gene/s providing resistance to this viral disease is suggested.

Keywords

Agroinfection

BCTIV

Resistance

Tomato

20.1001.1.22519041.2016.5.3.15.8

Akhtar, K. P., Hussain, M., Khan, A. I., Ahsanul Haq, M. and Mohsin Iqbal, M. 2004. Influence of plant age, whitefly population and cultivar resistance on infection of cotton plants by cotton leaf curl virus (CLCuV) in Pakistan. Field Crops Research, 86: 15-21.

Arunachalam, P., Radhakrishnan, V. V., Mathew, S. K. and Kumar, P. G. S. 2002. Reaction of bitter gourd genotypes against distortion mosaic virus. Vegetable Science, 29: 55-57.

Bian, X., Thomas, M. R., Rasheed, M. S., Saeed, M., Hanson, P., Barro, P. J. d. and Rezaian, M. A. 2007. A recessive allele (tgr-1) conditioning tomato resistance to geminivirus infection is associated with impaired viral movement. Phytopathology, 97: 930-937.

Bolok Yazdi, H., Heydarnejad, J. and Massumi, H. 2008. Genome characterization and genetic diversity of beet curly top iran virus: A geminivirus with a novel nonanucleotide. Virus Genes, 36: 539-545.

Brown, J. K., Fauquet, C. M., Briddon, R. W., Zerbini, M., Moriones, E. and Navas Castillo, J. 2012. Geminiviridae In: King, A. M. Q., Adams, M. J., Carstens, E. B. and Lefkowitz, E. J. (Eds.), Virus taxonomy: Ninth report of the international committee on taxonomy of viruses Elsevier, London. pp. 351-373.

Eini, O., Behjatnia, S. A. A., Dogra, S., Dry, I. B., Randles, J. W. and Rezaian, M. A. 2009. Identification of sequence elements regulating promoter activity and replication of a monopartite begomovirus-associated DNA β satellite. Journal of General Virology, 90: 253-260.

Eini, O., Sahraei, G.E., Behjatnia, S. A. A., 2016. Molecular characterization and construction of an infectious clone of a pepper isolate of Beet curly top Iran virus. Molecular Biology Research Communications, 5: 101-113.

Elmer, J. S., Brand, L., Sunter, G., Gardiner, W. E., Bisaro, D. M. and Rogers, S. G. 1988. Genetic analysis of the tomato golden mosaic virus II. The product of the AL1 coding sequence is required for replication. Nucleic Acids Research, 16: 7043-7060.

Fatahi, Z., Behjatnia, S. A. A., Afsharifar, A., Hamzehzarghani, H., Izadpanah, K. 2012. Screening of sugar beet cultivars for resistance to iranian isolate of beet severe curly top virus using an infectious clone of the virus. Iranian Journal of Plant Pathology 48: 111-121.

Friedmann, M., Lapidot, M., Cohen, S. and Pilowsky, M. 1998. A novel source of resistance to tomato yellow leaf curl virus exhibiting a symptomless reaction to viral infection. Journal of the American Society for Horticultural Science, 123: 1004-1007.

Gharouni Kardani, S., Heydarnejad, J., Zakiaghl, M., Mehrvar, M., Kraberger, S. and Varsani, A. 2013. Diversity of beet curly top iran virus isolated from different hosts in Iran. Virus Genes, 46: 571-575.

Hennegan, K. P. and Danna, K. J. 1998. PBin20: An improved binary vector for agrobacterium-mediated transformation. Plant Molecular Biology Reports, 16: 129-131.

Heydarnejad, J., HosseiniAbhari, E., Bolok Yazdi, H. R. and Massumi, H. 2007. Curly top of cultivated plants and weeds and report of a unique curtovirus from Iran. Journal of Phytopathology, 155: 321-325.

Jiang, Y.-X., Blas, C. d., Bedford, I., Nombela, G. and Muñiz, M. 2004. Effect of Bemisia tabaci biotype in the transmission of Tomato yellow leaf curl sardinia virus between tomato and common weeds. Spanish Journal of Agricultural Research, 2: 115-119.

Kanakala, S., Verma, H. N., Vijay, P., Saxena, D. R. and Malathi, V. G. 2013. Response of chickpea genotypes to agrobacterium-mediated delivery of Chickpea chlorotic dwarf virus (CpCDV) genome and identification of resistance source. Applied Microbiology and Biotechnology: 1-11.

Kheyr-Pour, A., Bendahmane, M., Matzeit, V., Accotto, G. P., Crespi, S. and Gronenborn, B. 1991. Tomato yellow leaf curl virus from sardinia is a whitefly- transmitted monoparatite geminivirus. Nucleic Acids Research, 19: 6763-6769.

Lapidot, M., Ben-Joseph, R., Cohen, L., Machbash, Z. and Levy, D. 2006. Development of a scale for evaluation of Tomato yellow leaf curl virus resistance level in tomato plants. Phytopathology, 96: 1404-1408.

Larsen, R. C. and Miklas, P. N. 2004. Generation and molecular mapping of a scar linked with the bct gene for resistance to Beet curly top virus in common bean. Phytopathology, 94: 230-325.

Lee, S., Stenger, D. C., Bisaro, D. M. and Davies, K. R. 1994. Identification of loci in arabidopsis that confer resistance to geminivirus infection. The Plant Journal, 6: 525-535.

Mason, G., Caciagli, P., Accotto, G. and Noris, E. 2008. Real-time PCR for the quantitation of Tomato yellow leaf curl Sardinia virus in tomato plants and in Bemisia tabaci. Journal of Virological Methods, 147: 282-289.

Montazeri, R., Shams-Bakhsh, M., Mahmoudi, S. B. and Rajabi, A. 2016. Evaluation of sugar beet lines for resistance to beet curly top viruses. Euphytica, 210: 31-40.

Rouhibakhsh, A., Priya, J., Periasamy, M., Haq, Q. M. I. and Malathi, V. G. 2008. An improved DNA isolation method and PCR protocol for efficient detection of multicomponents of begomovirus in legumes. Journal of Virological Methods, 147: 37-42.

Sabina, I., Munshi, A. D., Bikash, M., Ravinder, K. and Behera, T. K. 2010. Genetics of resistance in Luffa cylindrica Roem. against Tomato leaf curl new delhi virus. Euphytica, 174: 83-89.

Sahu, P., Sharma, N., Puranik, S., Muthamilarasan, M. and Prasad, M. 2014. Involvement of host regulatory pathways during geminivirus infection: A novel platform for generating durable resistance. Functional and Integrative Genomics, 1: 1-12.

Soleimani, R., Matic, S., Taheri, H., Behjatnia, S. A. A., Vecchiati, M., Izadpanah, K. and Accotto, G. P. 2013. The unconventional geminivirus Beet curly top Iran virus: Satisfying koch's postulates and determining vector and host range. Annals of Applied Biology, 162: 174-181.

Stenger, D., Revington, G., Stevenson, M. and Bisaro, D. 1991. Replicational release of geminivirus genomes from tandemly repeated copies: Evidence for rolling-circle replication of a plant viral DNA. Proceedings of the National Academy of Sciences, 88: 8029-8033.

Varsani, A., Navas-Castillo, N., Moriones, E., Hernandez-Zepeda, C., I., A., Brown, J. K., F., M. Z. and Martin, F. D. 2014. Establishment of three new genera in the family geminiviridae: Becurtovirus, Eragrovirus and Turncurtovirus. Archives of Virology, 159: 2193-2203.

Wang, H., de A. Gurusinghe, P. and Falk, B. W. 1999. Systemic insecticides and plant age affect beet curly top virus transmission to selected host plants. Plant Disease, 83: 351-355.

XML

PDF 544.26 K