Effect of bio-fertilizer on growth, development and nutrient content (leaf and soil) of Stevia rebaudiana Bertoni

10.48311/jcp.2015.1250
Volume 4, Issue 5
December 2015
Pages 691-704

Authors

1 Faculty of Agriculture, Science and Research Branch, Islamic Azad University, Tehran, Iran.

2 Department of Plant Patlology, Faculty of Agriculture, Tarbiat Modares University, P.O.Box: 14115-336, Tehran, Iran

Abstract
The aim of this study was to evaluate the effect of bio-fertilizers on growth and development of plants in pots containing (V) vermicompost and (M) cow manure: (soil amendments) and (S) garden soil separately and in combination. In the first experiment, treatments included S (control), SM (soil + 15%M + 0%V), SMV10 (soil + 15%M + 10%V), and SMV20 (soil + 15%M + 20%V) by dry weight (w/w). In the second experiment, mycorrhizae (Glomus mosseae and Piriformospora indica) were also added to the treatments. Plants and soils were evaluated 90 days after inoculation with fungi. At maturity, plant height and stem diameter, leaf area index (LAI), chlorophylls (a + b) content, root length, fresh and dry weights (aerial parts and root), and mineral nutrient content in plant leaves were measured. The obtained results indicated significant differences between treatments. Among all the treatments, interaction of soil amendments and fungus was significant for all traits. Almost the maximum amount of morphological traits was found in the combined applications of soil amendments and fungi. In particular, the highest weights were obtained in the SMV20 treatment inoculated with G. mosseae. Nutrient analysis of leaves, revealed maximum amounts of N, P and K in SMV10 and SMV20 treatments, and the highest amount of Ca and Mg was obtained in plants treated with SM and inoculated with P. indica in comparison to control plants. Also, the highest amount of phosphorous in the soil was obtained when SMV20 treatment was inoculated with P. indica. It seems that the combination of biofertilizers is the best result for the stevia plant growth..

Keywords

Atiyeh, R. M., Subler, S., Edwards, C. A. and Metzger, J. 1999. Growth of tomato plants in horticulture potting media amended with vermicompost. Pedobiologia, 43: 724-728.
Arancon, N., Edwards, C. A., Bierman, P., Welch, C. and Metzger, J. D. 2004. Influences of vermicomposts on field strawberries: 1. Effects on growth and yields. Bioresource Technology, 93: 145-153.
Arguello, J. A., Ledesma, A., Nunez, S. B., Rodriguez, C. H. and Goldfarb, M. D. D. 2006. Vermicompost effects on bulbing dynamics, nonstructural carbohydrate content, yield, and quality of Rosado Paraguay garlic bulbs. Horticultural Science, 41: 589-592.
Atiyeh, R. M., Arancon, N., Edwards, C. A. and Metzger, J. D. 2002. The influence of earthworm-processed pig manure on the growth and productivity of marigolds. Bioresource Technology, 81: 103-108.
Azizi, M., Rezwanee, F., Khayat, M. H., Lackzian, A. and Neamati, H. 2008. The effect of different levels of vermicompost and irrigation on morphological properties and essential oil content of German chamomile (Matricaria recutita). Journal of Medicinal and Aromatic Plants, 24: 82-93.
Brandle, J. E., Starrratt, A. N. and Gijen, M. 1998. Stevia rebaudiana: its agricultural, biological and chemical properties. Canadian Journal of Plant Science, 78: 527-536.
Bano, K., Kale, R. D. and Satyavathi, G. P. 1993. Vermicompost as fertilizer for ornamental plants. IV National Symposium Soil, Biology. Ecology. Bangalore, pp. 165-168.

Brundrett, M., Bougher, N., Dell, B., Grove, T. and Malajczuk, N. 1996. Working with mycorrhiza in forestry and agriculture. Australian Centre for International Agricultural Research Monograph, 32: 374.


Chapman, H. D. and Pratt, P. F. 1961. Methods for determination of phosphorus: Methods of analysis for soils, plants and waters. Division of Agricultural Sciences, University of California, Riverside, pp. 184-203.
Chaturvedula, V. S. P., Rhea, J., Milanowski, D., Mocek, U. and Prakash, I. 2011. Two minor diterpene glycosides from the leaves of stevia rebaudiana. National Product Communication, 6: 175-8.
Chaudhry, M. A., Rehman, A., Naeem, M. A. and Mushtaq, N. 1999. Effect of organic and inorganic fertilizers on nutrient contents and some properties of eroded loess soil. Pakistan Journal of Soil Science, 16: 63-68.
Darise, M., Kohda, H., Mizutani, K., Kasai, R. and Tanaka, O. 1983. Chemical constituents of flowers of stevia rebaudiana Bertoni. Agricultural and Biological Chemistry, 47: 133-135.
Darzi, M. T., Ghalavand, A., Rejali, F. and Sephidkon, F. 2007. Effects of Biofertilizers Application on yield and yield components in fennel (Foeniculum vulgare Mill.). Iranian Journal of Medicinal and Aromatic Plants, 22: 276-292.
Darzi, M. T., Ghalavand, A. and Rejali, F. 2008. Effect of mycorrhiza, vermicompost and phosphate biofertilizer application on flowering biological yield and root colonization in fennel (Foeniculum vulgare Mill.). Iranian Journal of Crop Sciences, 10: 88-109.
Das, K., Dang, R., Shivananda, T. N. and Sekeroglu. N. 2007. Influence of bio-fertilizers on the biomass yield and nutrient content in Stevia rebaudiana Bert. grown in Indian subtropics. Journal of Medicinal Plants Research, 1: 005-008.
Edwards, C. A. and Burrows, I. 1988. The potential of earthworm composts as plant growth media. In: Neuhauser, C.A. (Ed.), Earthworms in Environmental and Waste Management. SPB Academic Publishing, The Hague, the Netherlands, pp. 211-220.
Eghball, B., Ginting, D., Gilley, J. E. 2004. Residual effects of manure and compost applications on corn production and soil properties. Agronomy Journal, 96: 442-7.
Fernandez, R., Scull, R., Gonzales, J. L., Crespo, M., Sanchez, E. and Carball, C. 1993. Effect of fertilization on yield and quality of Matricaria reculita L. (Chamomile). Aspects of mineral nutrition of the crop. Memorias 11th Congress Latino Americano de la Ciencia del Suelo. 2ed Congresso Cubcno de la Ciencia del Suelo, Berlin, Germany, p, 891-894.
Frankenberger, J. R. and Arshad, W. T. 1995. Phytohormones in Soils: Microbial Production and Function. Marcel and Decker, New York, 503 p.
Fronza, D. and Folegatti, M. V. 2003. Water consumption of the Stevia (Stevia rebaudiana Bert.) crop estimated through microlysimiter. Scientia. Agricola, 60-80.
Grappelli, A., Tomati, U. and Galli, E. 1985. Earthworm casting in plant propagation, Horticulture. Science, 20: 874-876.
Geuns, J. M. C. 2003. Molecules of interest stevioside. Phytochemistry, 6: 913-921.
Geuns, J. M. 2010. Stevia and steviol glycosides. Euprint, Heverlee.
Gutiérrez-Miceli, F., Santiago-Boraz, J., Molina, J. A. M., Nafat, C. C., Abdul-Archila, M., Llaven, M. A. O., Rincón-Rosales, R. and Dendooven, L. 2007. Vermicompost as a soil supplement to improve growth, yield and fruit quality of tomato (Lycopersicum esculentum). Bioresource Technology, 98: 2781-2786.
Harrier, L. A. and Sawczak, J. 2000. Detection of the 3-phosphoglycerate kinase protein of Glomus mosseae. Mycorrhiza, 10: 81-86.
Hazarika, D. K., Taluk Dar, N. C., Phookan, A. K., Saikia, U. N., Das, B. C. and Deka, P. C. 2000. Influence of vesicular arbascular mycorrhizal fungi and phosphate solubilizng bacteria on nursery establishment and growth of tea seedlings in assam. Symposium No. 12, Assam Agricultural University, Jorhat-Assam, India.
ISRIC 1986 Procedure for Soil Analysis. International soil reference and information center (ISRIC), Washington Agriculture University.
Kale, R. D., Bano, K., Sreenivasa, M. N. and Bagyaraj, D. J. 1987. Influence of worm cast on the growth and mycorrhizal colonization of two ornamental plants. South Indian Horticulture, 35: 433-437.
Kale, R. D. 1998. Earthworm: Cinderella of organic farming. Prism Books. Bangalore. India.
Kumar, S., Choudhary, G. R. and Chaudhari, A. C. 2002. Effects of nitrogen and biofertilizers on the yield and quality of coriander (Coriandrum sativum L.). Annals of Agriculture Research, 23: 634-637.
Kumari, R., Kishan, H., Bhoon, Y. K. and Varma, A. 2003. Colonization of cruciferous plants by piriformospora indica. Current. Science, 85: 1672-1674.
Mallanagouda, B, 1995. Effects of N, P, K and fym on growth parameters of onion, garlic and coriander. Journal of Medicinal and Aromatic Plant Science, 4: 916-918.
Mohammadi Goltapeh, E. Rezaee Danesh, Y. and Varma, A. 2013. Fungi as Bioremediators, Soil Biology Springer.
Nadri, M. 2012. zagrosdaroo. Available on: http://www.bsmt. ir
Ntanos, D. A. and Koutroubas, S. D. 2002. Dry matter and N accumulation and translocation for Indica and Japonica rice under Mediterranean conditions Field Crops. Research, 74: 93-101.
Ohta, M., Sasa, S., Inoue, A., Tamai, T., Fujita, I., Morita, K. and Matsuura, F. 2010. Characterization of novel steviol glycosides from leaves of Stevia rebaudiana morita. Journal of Applied Glycoscience, 57: 199-209.
Padmapriya, S. and Chezhiyan, N. 2009. Effect of shade, organic, inorganic and biofertilizers on morphology, yield and quality of turmeric. Indian Journal of Horticulture, 66 (3): 333-339.
Pandey, R. 2005. Management of Meloidogyne incognita in Artemisia pallens with bio-organics. Phytoparasitica, 33: 304-308.
Paterson, E. 2003. Importance of rhizodeposition in the coupling of plant and microbial productivity. European Journal of soil Science, 54: 741-750.
Rakesh, J. and Adarsh, P. V. 2010. Effect of vermicompos on growth, yield and quality of tomato (Lycopersicum esculentum L). Africa Journal of Basic and Applied Sciences. 2: 117-123.
Rai, M., Acharya, D., S ingh, A. and Varma, A. 2001. Positive growth responses of the medicinal plants Spilanthes calva and Withanio somnifera to inoculation by piriformospra indica in a field trail. Mycorrhiza, 11: 123-128.
Ratti, N., Kumar, S., Verma, H. N. and Gautam, S. P. 2001. Improvement in bioavailability of tricalcium phosphate to Cymbopogon martini var. Motia by rhizobacteria, AMF and Azospirillum inoculation. Microbiological. Research, 156: 145-149.
Roy, D. K. and Singh, B. P. 2006. Effect of level and time of nitrogen application with and without vermicompost on yield, yield attributes and quality of malt barley (Hordeumvulgare). Indian Journal of Agronomy, 51: 40-42.
Sahay, N. S. and Varma, A. 1999. Piriformospora indica: a new biological hardening tool for micropropagated plants. FEMS Microbiology letters, 181: 297-302.
Schussler, A., Schwarzott, D. and Walker, C. 2001. A new fungal phylum, the Glomeromycota: phylogeny and evolution. Mycological Research, 105: 1413-1421.
Singh, A., Sharma, J., Rexer, K. H. and Varma, A. 2000. Plant productivity determinants beyond minerals, water and light, Piriformospora indica: a revolutionary plant growth promoting fungus. Current Science, 79: 1548-1554.
Tomati, U., Grapppelli, A. and Galli, E. 1988. The hormone like effect of earthworm casts on plant growth. Biology an Fertility of Soils. 5: 288-294.
Tejada, M. and Gonzalez, J. 2006. Effect of foliar application of beet vinasse on maize yield. Biological Agriculture and Horticulture, 24: 197-214.
Tejada, M., Gonzalez, J., Hernandez, M. and Garcia, C. 2007. Agricultural use of leachates obtained from two different vermicomposting processes. Bioresource Technology, 99: 6228-6232.
Vadiraj, B. A., Siddagangaiah, A. and Poti, N. 1998. Effect of vermicompost on the growth and yield of turmeric. South Indian Horticulture, 46: 176-179.
Varma, A., Singh, A., Sudha, N., Sahay, S., Sharma, J., Roy, A., Kumari, M., Rana, D., Thakran, S., Deka, D., Bharti, K., Franken, P., Hurek, T., Blechert, O., Rexer, K-H., Kost, G., Hahn, A., Hock, B., Maier, W., Walter, M., Strack, D and Kranner, I. 2001. Piriformospora indica: a cultivable mycorrhiza-like endosymbiotic fungus. Mycota IX, Springer Series, Germany, pp: 123-150
Varma, A., Verma, S., Sudha sahay, N. and Franken, P. 1999. Piriformospora indica, a cultivable plant growth-promoting root endophyte. Applied and Environmental Microbiology, 65: 2741-2744.
Waller, F., Achatz, B., Baltruschat, H. and Fodor, J. 2005. The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield. Proceedings of the national Academy of Sciences of United State of America, 38: 13386-13391.
Walinga, I., Van Vark, W., Houba, V. I. G. and Vander Lee. J. J. (1989). Plant Analysis, Procedures Part 7. Department of Soil Science and Plant Nutrition, Wageningen Agriculture University, 197-200.