Bipolaris species associated with rice plant: pathogenicity and genetic diversity of Bipolaris oryzae using rep-PCR in Mazandaran province of Iran

Nazari, Somayeh; Javan-Nikkhah, Mohammad; Fotouhifar, Khalil-Berdi; Khosravi, Vahid; Alizadeh, Alireza

doi:10.48311/jcp.2015.1233

Bipolaris species associated with rice plant: pathogenicity and genetic diversity of Bipolaris oryzae using rep-PCR in Mazandaran province of Iran

Authors

Somayeh Nazari

Mohammad Javan-Nikkhah

Khalil-Berdi Fotouhifar

Vahid Khosravi

Alireza Alizadeh

Department of Plant Protection, Faculty of Agricultural Science and Natural Resources, University of Tehran, Karaj, Iran.

10.48311/jcp.2015.1233

Abstract

Ninety one monoconidial Bipolaris isolates were obtained from lesions on different parts of rice in different locations of Mazandaran province during the summer of 2009. Bipolaris species were identified using morphological features such as color and shape of colony and color and size of conidia and conidiophores. The isolates were separated into two species; 85 (93.4%) isolates belonged to Bipolaris oryzae and the remaining 6 (6.6%) isolates to Bipolaris cynodontis. Therefore B. oryzae is regarded as the major cause of rice brown spot disease in Mazandaran province. In order to analyze genetic diversity among B. oryzae isolates, 71 isolates were subjected to fingerprinting analysis by rep-PCR using BOX and REP primers. In cluster analysis, 15 clonal lineages and 54 haplotypes were identified. The largest clonal lineage contained with 36 haplotypes was the most common lineage. These results also indicate a relatively high level of genetic diversity among B. oryzae isolates. Also, pathogenicity test of a few B. oryzae isolates (12 isolates) was conducted under greenhouse condition and showed that those isolates were pathogenic to rice seedlings of cv. Tarom. All isolates produced some leaf spots 24 h after inoculation.

Keywords

Bipolaris

Rice

Genetic diversity

REP and Box Primers

Pathogenicity test

Mazandaran Province