PCR-based characterization of Puccinia graminis f. sp. tritici race TTSSK from Iran

Mojerlou, Shideh; Safaie, Naser; Abasi-Moghadam, Ahmad; Shams-Bakhsh, Mashoud

doi:10.48311/jcp.2015.1217

PCR-based characterization of Puccinia graminis f. sp. tritici race TTSSK from Iran

10.48311/jcp.2015.1217

Volume 4, Issue 3
September 2015

Pages 321-328

Authors

S. Mojerlou ¹

N. Safaie ¹

A. Abasi-Moghadam ²

M. Shams-Bakhsh ¹

¹ Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

² Department of Genetics and National Plant Gene-Bank of Iran, Seed and Plant Improvement Institute Karaj, Iran.

Abstract

Stem rust caused by Puccinia graminis f. sp. tritici (Pgt) is one of the destructive diseases of wheat in the world. The fungal pathogen can infect 365 different grass and more than 70 Berberis species. DNA sequences for the ribosomal RNA internal transcribed spacer (ITS) have proven suitable to explore relationships at the species and subspecies levels. An isolate of Pgt which was collected from Iran and designated as TTSSK was used in this study. Three samples of the isolate were used. ITS region of the samples was amplified and sequenced. Consensus tree based on Maximum Parsimony clustering method was produced by Mega 6.0. Iranian isolate of TTSSK was placed in a clade with P. graminis which was isolated from Berberis sp. and Pgt isolate from Tajikistan. The results showed that more than one conserved genomic regions would be informative to identify phylogenetic relationship of Iranian Pgt isolates and samples from different parts of the world. Complementary studies with more sequence data from other genome loci are in progress.

Keywords

ITS

maximum parsimony

phylogenetic relation

stem rust

Ug99 race group