Journal of Crop Protection

Characterization of digestive α-amylase in the midgut of willow leaf beetle Plagiodera versicolora (Coleoptera: Chrysomelidae)

10.48311/jcp.2014.1110
Volume 3, Issue 2
June 2014
Pages 245-254
Journal of Crop Protection

Authors

M. Shabarari 1
B. Naseri 1
A. Zibaee 2
J. Hajizadeh 2

1 Department of Plant Protection, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, 56199-11367, Ardabil, Iran.

2 Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, 41635-1314, Rasht, Iran.

Abstract
Willow leaf beetle, Plagiodera versicolora is an important pest of willow trees that feeds on the leaves both as larvae and as adults. -Amylases (EC 3.2.1.1) are the major insect digestive enzymes that catalyze the endohydrolysis of long -1,4-glucan chains such as starch and glycogen. In the current study, -amylase activity was studied in the midgut of larvae and adults of P. versicolora. Amylase activity in the midgut of larvae and adults was 0.6807 and 0.1162 µmol/min/mg protein, respectively. The optimal pH for -amylase activity of larvae and adults was 4 and 8, respectively and the optimal temperature for both was 35 °C. The enzyme activity of larvae was inhibited by the addition of Na2+, K+ and Zn2+. K+ (at 5 mmol) had the most positive effect on α-amylase activity in adults. EGTA had significant influence on decreasing the enzyme activity in larvae. EDTA had the most effect on increasing the activity of the enzyme in adults. Plant amylase inhibitors play important role against insect pests. Therefore, the characterization of digestive enzymes and the examination of inhibitors on the enzyme activity could be useful in tackling insect pests.

Keywords

Plagiodera versicolora
midgut
α- amylase
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Zibaee, A. and Hajizadeh, J. 2013. Proteolytic activity in Plagiodera versicolora Laicharting (Coleoptera: Chrysomelidae): Characterization of digestive proteases and effect of host plants. Journal of Asia-Pacific Entomology, 16: 329-334.


Zibaee, A., Bandani, A., R., Kafil, M. and Ramzi, S. 2008. Characterization of α-amylase in the midgut and the salivary glands of rice striped stem borer, Chilo suppressalis Walker (Lepidoptera: Pyralidae). Journal of Asia-Pacific Entomology, 11: 201-205.

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