Journal of Crop Protection

Occurrence of deoxynivalenol producing isolates of Fusarium graminearum species complex associated with head blight of wheat in Moghan area

10.48311/jcp.2014.1099
Volume 3, Issue 2
June 2014
Pages 113-123
Journal of Crop Protection

Authors

M. Davari 1
N. Safaie 2
M. Darvishnia 3
R. Didar Taleshmikaeel 4

1 Department of Plant Protection, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran.

2 Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

3 Department of Plant Protection, Faculty of Agriculture, University of Lorestan, Khoram Abad, Iran.

4 Department of Plant Production, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

Abstract
The severe epidemics of Fusarium head blight (FHB) as a devastating disease of cereal crops has occurred on wheat in North and Northwest Iran in recent years. The in vitro production of Deoxynivalenol (DON) was qualitatively evaluated in 41 Fusarium isolates collected from wheat heads associated with the scab disease, in Moghan area/Northwest Iran. Infected wheat heads were collected during 2004-2007. The isolation of causal agents was carried out using standard methods. According to morphological characteristics and using valid descriptions, all isolates belonged to Fusarium graminearum species complex and F. culmorum of which the former was dominant. In order to evaluate the potential of DON production in isolates, this mycotoxin was extracted and qualitatively examined by TLC method. The quantification of DON was achieved using HPLC method. TLC results indicated that 54.5% of studied isolates produced DON but there was no significant relationship between this property and cultivars or sub-regions or years. Also based on HPLC analysis, maximum content of DON was detected in F. graminearum isolated from cv. Izen green from Moghan Agro-industry company fields in 2004 at the rate of 5827.11 µgkg-1. The results of present study show that DON mycotoxin is produced at various contents by F. graminearum isolates on different cultivars and from different origins/ years. Since DON plays a role in pathogenesis and is of paramount importance in contamination of wheat grains, these results give a better insight into the significance of this disease in Northwest Iran.

Keywords

Chemotype
DON
F. graminearum sensu lato
Fusarium head blight
Northwest Iran
Alizadeh, A., Etaati, M., Safaie, N. and Saidi, A. 2003. A sensitive bioassay method for evaluation of zearalenone production by Fusarium graminearum isolates the causal agent of wheat scab. Iranian Journal of Plant Pathology, 39: 39-44.
Alvarez, C. L., Azcarate M. P. and Fernández Pinto, V. 2009. Toxigenic potential of Fusarium graminearum sensu stricto isolates from wheat in Argentina. International Journal of Food Microbiology, 135: 131-135.
Bai, G. H. and Shaner, G. 1994. Scab of wheat: Prospects for control. Plant Disease, 78: 760-766.
Bottalico, A. and Perrone, G. 2002. Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology, 108: 611-624.
Boutigny, A. L., Ward, T. J., Van Coller, G. J., Flett, B., Lamprecht, S. C., O'Donnell, K. and Viljoen, A. 2011. Analysis of the Fusarium graminearum species complex from wheat, barley and maize in South Africa provides evidence of species-specific differences in host preference. Fungal Genetics and Biology, 48: 914-920.
Chehri, K., Maghsoudlou, E., Asemani, M. and Mirzaei, M. R. 2011. Identification and pathogenicity of Fusarium species associated with head blight of wheat in Iran. Pakistan Journal of Botany, 43: 2607-2611.
Cumagun C. J. R. and Miedaner, T. 2004. Segregation for aggressiveness and deoxynivalenol production of a population of Gibberella zeae causing head blight of wheat. European Journal of Plant Pathology, 110: 789-99.
Cundilefe, E. and Davis J. E. 1997. Inhibition, elongation and termination of eukaryotic protein Synthesis by trichothecens fungal toxin. Antimicrobial Agents and Chemotherapy, 11: 491- 499.
Davari, M., Didar, R. and Hajieghrari, B. 2006. Wheat Fusarium head blight and identification of dominant species in Moghan area/Iran. Communications in Agricultural and Applied Biological Sciences, 71: 1391-1397.
Davari, M., van Diepeningen, A. D., Babai-Ahari, A., Arzanlou, M., Najafzadeh, M. J., van der Lee, T. A. J. and de Hoog, G. S. 2012. Rapid identification of Fusarium graminearum species complex using Rolling Circle Amplification (RCA). Journal of Microbiological Methods, 89: 63-70.
Davari M., Wei, S. H., Babay-Ahari, A. Arzanlou, M., Waalwijk, C., van der Lee, T. A. J., Zare, R., Gerrits van den Ende, A. H. G., de Hoog, G. S. and van Diepeningen, A. D. 2013. Geographic differences in trichothecene chemotypes of Fusarium graminearum in the Northwest and North of Iran. World Mycotoxin Journal, 6: 137-150.
Desjardins, A. E. and Hohn, T. M. 1997. Mycotoxins in Plant Pathogenesis. Molecular Plant-Microbe Interactions, 10: 147-152.
Ehling, G., Cockbur, A., Snowdon, P. and Buchhause, H. 1997. The significance of the Fusarium toxin deoxynivalenol for human and animal health. Cereal Research Communications, 25: 433-447.
Eslahi, M. R., Dadrezaie, T. and Farokhinejad, R. 2008. Identification of wheat Fusarium head blight and determination of their pathogenecity in Khuzestan province. Pajouhesh and Sazandegi, 81: 61-66 (in Persian with English Abstract).
Gale, L. R., Ward, T. J., Balmas, V. and Kistler, H. C. 2003. Detection of distinct subpopulations of Fusarium graminearum lineage 7 in the US. 2003National Fusarium Head Blight Forum Proceeding, p. 139.
Geraldo1, M. R. F, Tessmann, D. J. and Kemmelmeier, C. 2006. Production of mycotoxins by Fusarium graminearum isolated from small cereals (wheat, triticale and barley) affected with scab disease in southern Brazil. Brazilian Journal of Microbiology, 37: 58-63
Golzar, H., Foroutan, A. and Ershad, D. 1998. Studies on fusarium species causing head blight of wheat and sources of resistance of F. graminearum in Golestan and Mazandaran. Iranian Journal of Plant Pathology, 34: 48-52.
Goswami, R. S. and Kistler, H. C. 2004. Heading for disaster: Fusarium graminearum on cereal crops. Molecular Plant Pathology, 5: 515-525.
Haratian, M., Sharifnabi, B., Alizadeh, A. and Safaie, N. 2008. PCR analysis of the Tri13 gene to determine the genetic potential of Fusarium graminearum isolates from Iran to produce nivalenol and deoxynivalenol. Mycopathologia, 166: 109-16.
Islami, F., Kamangar, F., Nasrollahzadeh, D., Moller, H., Boffetta, P. and Malekzadeh, R. 2009. Oesophageal cancer in Golestan Province, a high-incidence area in northern Iran, A review. European Journal of Cancer, 45: 3156-3165.
Jennings, P. M., Caotes, E., Walsh, K., Turner, J. A. and Nichoson, P. 2004. Determination of deoxynivalenol and nivalenol-producing chemotypes of Fusarium graminearum isolates from wheat crops in England and Wales. European Journal of Plant Pathology, 53: 643-652.
JECFA. 2010. Summary and conclusions of the 72nd meeting of the Joint FAO/WHO Expert Committee on Food Additives (JECFA/72/SC). FAO, Rome, Italy. Available at: www.who. int/entity/ foodsafety/chem/summary72_rev.pdf (accessed December 2012).
Kamangar, F., Chow, W. H., Abnet, C. and Dawsey, S. 2009. Environmental causes of esophageal cancer Gastroenterology. Gastroenterology Clinics of North America, 38: 27-57.
Karami-Osboo, R. and Mirabolfathi, M. 2008. Natural zearalenone contamination of wheat from Golestan Province, Northern Iran. Iranian Journal of Plant Pathology, 44: 60.
Lauren, D. R. and Agnew, M. P. 1991. Multitoxin screening method for Fusarium mycotoxins grain. Journal of Agricultural and Food chemistry, 39: 502-507.
Leslie, J. F. and Summerell, B. A. 2006. The Fusarium laboratory manual. Blackwell Publishing Ltd. Oxford, UK. 388 pp.
Marasas, W. F. O., van Rensburg, S. J. and Mirocha, C. J. 1979. Incidence of Fusarium species and the mycotoxins, deoxynivalenol and zearalenone, in corn produced in esophageal cancer areas in Transkei. Journal of Agricultural and Food chemistry, 27: 1108-1112.
Mesterhazy, A., Bartok, T., Mirocha, C. G. and Komoroczy, R. 1999. Nature of wheat resistance to Fusarium head blight and the role of deoxynivalenol for breeding. Plant Breeding, 118: 97-110
Miller, J. D., Greenhalgh, R., Wang, Y. Z. and Lu, M. 1991. Trichothecene chemotypes of three Fusarium species. Mycologia, 83: 121-130.
Moosawi-Jorf, S. A., Farrokhi-Nejad, R., Azimi, S. and Afarin, S. 2007. Study of Fusarium head blight of wheat in Khuzestan Province in Iran and reporting of Fusarium xylarioides as a new causal agent for disease. Journal of Agronomy, 6: 212-215.
Nelson, P. E., Toussoum, T. A. and Marasas, W. F. O. 1983. Fusarium species, an illustrated manual for identification. The Pennsylvania State University Press. Press Park and London. 193 pp.
O'Donnell, K., Ward, T. J., Geiser, D. M., Kistler, H. C. and Aoki, T. 2004. Genealogical concordance between the mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade. Fungal Genetics and Biology, 41: 600-623.
Parry, D., Jenkinson, P. and Mc Leo, D. L. 1995. Fusarium ear blight (Scab) in small grain cereales–A review. Plant Pathology, 44: 207-238.
Placinta, C. M. D., Mello, J. P. F. and Macdonald, A. M. C. 1999. A review of Worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Animal Feed Science and Technology, 78: 21-37.
Proctor, R. H., Desjardins, A. E., Mc Cormick, S. P., Plattner, R. D., Alexander, N. J. and Brown, D. W. 2002. Genetic analysis of the role of trichothecene and fumonisin mycotoxins in the virulence of Fusarium. European Journal of Plant Pathology, 108: 691-698.
Prodi, A., Tonti, S., Nipoti, P., Pancaldi, D. and Pisi, A. 2009. Identification of deoxynivalenol and nivalenol producing chemotypes of Fusarium graminearum isolates from durum wheat in a restricted area of Northern Italy. Journal of Plant Pathology, 91: 727-731.
Reynoso, M. M., Ramirez, M. L., Torres, A. M. and Chulze, S. N. 2011. Trichothecene genotypes and chemotypes in Fusarium graminearum strains isolated from wheat in Argentina. International Journal of Food Microbiology, 145: 444-448.
Rodrigues, I. and Naehrer, K. 2012. A three-year survey on the worldwide occurrence of mycotoxins in feedstuffs and feed. Toxins, 4: 663-675.
Sadjadi, A., Malekzadeh, R., Derakhshan, M. H., Sepehr, A., Nouraie, M., Sotoudeh, M., Yazdanbod, A., Shokoohi, B., Mashayekhi, A., Arshi, S., Majidpour, A., Babaei, M., Mosavi, A., Mohagheghi, M. A. and Alimohammadian, M. 2003. Cancer occurrence in Ardabil: results of a population based cancer registry from Iran. International Journal of Cancer, 107: 113-118.
Sarver, B. A. J., Ward, T. J., Gale, L. R., Broz, K., Kistler, H. C., Aoki, T., Nicholson, P., Carter, J. and O’Donnell, K. 2011. Novel Fusarium head blight pathogens from Nepal and Louisiana revealed by multilocus genealogical concordance. Fungal Genetics and Biology, 48: 1096-1107.
SAS Institute 1999. SAS/STAT User’s Guide, Version 7-1. SAS Institute Inc, Cary, North Carolina.
Starkey, D. E., Ward, T. J., Aoki, T., Gale, L. R., Kistler, H. C., Geiser, D. M., Suga, H., Tóth, B., Varga, J. and O'Donnell, K. 2007. Global molecular surveillance reveals novel Fusarium head blight species and trichothecene toxin diversity. Fungal Genetics and Biology, 44: 1191-1204.
Toth, B., Mesterhazy, A., Horvath, Z., Bartok, T., Varga, M. and Varga, J. 2005. Genetic variability of central European isolates of the Fusarium graminearum species complex. European Journal of Plant Pathology, 113: 35-45.
Turner, P. C., Gong, Y. Y., Pourshams, A., Jafari, E., Routledge, M. N., Malekzadeh, R., Wild, C. P., Boffetta, P. and Islami, F. 2012. A pilot survey for Fusarium mycotoxin biomarkers in women from Golestan, Northern Iran. World Mycotoxin Journal, 5: 195-199.
van Egmond, H. P., Schothorst, R. C. and Jonker, M. A. 2007. Regulations relating to mycotoxins, perspectives in a global and European context. Analytical and Bioanalytical Chemistry, 389: 147-157.
Xu, X. M., Parry, D. W., Nicholson, P., Thomsett, M. A., Simpson, D., Edwards, S. G., Cooke, B. M., Doohan, F. M., Brennan, J. M., Moretti, A., Tocco, G., Mule, G., Hornok, L., Giczey, G. and Tatnell, J. 2005. Predominance and association of pathogenic fungi causing Fusarium ear blight in wheat in four European countries. European Journal of Plant Pathology, 112: 143-154.
Zamanizadeh, H. R. and Khorsandi, H. 1995. Occurrence of Fusarium species and their mycotoxin in wheat in Mazandaran province, Iran. Iranian Journal of Plant Pathology, 31: 23-37.
Zhang, H., Van der Lee, T., Waalwijk, C., Chen, W., Xu, J., Jin Xu, J. S., Zheng, Y. and Feng, J. 2012. Population analysis of the Fusarium graminearum species complex from wheat in China show a shift to more aggressive isolates. PLoS One 7 (2): e31722.

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