Laboratory evaluation of Metarhizium anisopliae (Metschnikoff) for controlling Amitermes vilis (Hagen) and Microcerotermes gabrielis (Weidner) (Isoptera: Termitidae)

Rahimzadeh, Alireza; Rashid, Marziyeh; Sheikhi Garjan, Aziz; Naseri, Bahram

doi:10.48311/jcp.2012.1034

Laboratory evaluation of Metarhizium anisopliae (Metschnikoff) for controlling Amitermes vilis (Hagen) and Microcerotermes gabrielis (Weidner) (Isoptera: Termitidae)

Authors

Alireza Rahimzadeh ¹

Marziyeh Rashid ²

Aziz Sheikhi Garjan ³

Bahram Naseri ⁴

¹ Medical Department, Tarbiat Modares University, Tehran, Iran

² Plant Protection Department, Aboureihan Campus, University of Tehran, Tehran, Iran

³ Iranian Research Institute of Plant Protection, Tehran, Iran

⁴ Department of Plant Protection, Faculty of Agriculture, University of Mohaghegh Ardabili, Ardabil, Iran

10.48311/jcp.2012.1034

Abstract

Subterranean termites are one of the most important pests of buildings, historic monuments and agricultural crops in some parts of Iran. Using entomopathogenic fungi as microbial insecticides is usually a part of biological control and insect pest management. The pathogenicity of entomopathogenic fungus, Metarhizium anisopliae (Metschnikoff) Sorokin (DEMI 001) isolated from Rhynchophorus ferrugineus (Oliver) was compared against two subterranean termites, Amitermes vilis (Hagen) and Microcerotermes gabrielis (Weidner) under laboratory conditions. Suspensions of the fungus spores at five concentrations of 10¹, 10², 10³, 10⁴, 10⁶ spores ml^-1 were prepared to define LC50 and LT50. To determine LC50 and LT50 of M. anisopliae, bioassays were carried out on worker casts of both termite species. LC50 values for A. vilis and M. gabrielis were 8.5 × 10³and 0.2 × 10²spores ml^-1, respectively. LT50 value for M. gabrielis was shorter than that of A. vilis at all five concentrations tested. According to the results of the bioassay, M. anisopliae was more effective for controlling M. gabrielis than that for A. vilis.

Keywords

Amitermes vilis

Microcerotermes gabrielis

Metarhizium anisopliae

LC50 and LT50