Volume 10, Issue 2 (2021)
JCP 2021, 10(2): 391-399 |
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Nazari F, Safaie N, Momeni H. Evaluation of fluorescence-activated cell sorting technology in agrobacterium biocontrol. JCP 2021; 10 (2) :391-399
URL: http://jcp.modares.ac.ir/article-3-47366-en.html
URL: http://jcp.modares.ac.ir/article-3-47366-en.html
1- Department of Plant Disease Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension, Tehran, Iran. , fahimehnazari236@gmail.com
2- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
3- Department of Plant Disease Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension, Tehran, Iran.
2- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
3- Department of Plant Disease Research, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension, Tehran, Iran.
Abstract: (1721 Views)
Fluorescence-activated cell sorting (FACS) as a novel and sensitive technology was used to evaluate the biocontrol efficiency of Bacillus subtilis against Agrobacterium tumefaciens, a very destructive plant pathogen. The combination of two methods of culturing and cell sorting by FACS technology was used to distinguish a rapid and accurate method in monitoring the biocontrol effect of Bacillus (ATCC21332) on Agrobacterium (IBRC-M10701 and AGL1),. The culture method indicated that the B. subtilis could suppress A. tumefaciens in vitro and in vivo. We used a green fluorescent protein (GFP), reporter, to flow cytometric analysis using FACS. The mean of GFP expression levels was significantly reduced to 17.98, 16.48, and 11.27% in treatments 24, 48, and 72 h post-treatment; however, it was 31.57, 26.06, and 23.98% in the nontreated ones. The experiments demonstrated a positive biocontrol effect of Bacillus against Agrobacterium. Overall, our findings may provide a basis for improving the new rapid biocontrol agent detection method based on FACS.
Article Type: Original Research |
Subject:
Biocontrol of Plant Diseases
Received: 2020/11/4 | Accepted: 2021/04/4 | Published: 2021/04/21
Received: 2020/11/4 | Accepted: 2021/04/4 | Published: 2021/04/21
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