Efficacy of mixture of emamectin benzoate with some insecticides on the mortality and esterase activity of fourth instar larvae of Tuta absoluta (Lepidotera: Gelechiidae)

Taleh, Mohsen; Rafiee Dastjerdi, Hooshang; Naseri, Bahram; Sheikhi Garjan, Aziz; Talebi Jahromi, Khalil

doi:10.48311/jcp.2020.1511

Efficacy of mixture of emamectin benzoate with some insecticides on the mortality and esterase activity of fourth instar larvae of Tuta absoluta (Lepidotera: Gelechiidae)

https://doi.org/10.48311/jcp.2020.1511

Volume 9, Issue 4
December 2020

Pages 699-709

Document Type : Original Research

Authors

Mohsen Taleh ¹

Hooshang Rafiee Dastjerdi ¹

Bahram Naseri ¹

Aziz Sheikhi Garjan ²

Khalil Talebi Jahromi ³

¹ Departmant of Plant Protection, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.

² Iranian Research Institute of Plant Protection, Tehran, Iran.

³ Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.

Abstract

The tomato leafminer, Tuta absoluta Meyrick (Lepidoptera: Gelechiidae) is one of the most damaging tomato pests in the world and in Iran. The toxicity of acetamiprid, eforia (thiamethoxam + lambda-cyhalothrin) and hexaflumuron alone and in mixture with emamectin benzoate was studied against 4^th-instars of Tuta absoluta (Meyrick) at 25 ± 2 °C, 65 ± 5% RH and 16:8 (L:D) h. Moreover, the mixtures of examined insecticides with emamectin benzoate at LC₁₅:LC₁₅ratio were assessed on the general esterase enzyme activity and total protein content of 4^th-instars. The highest toxicity was found for emamectin benzoate after 72 h (LC₅₀ = 0.48 mg a.i./l), followed by acetamiprid (LC₅₀ = 46.94 mg a.i./l), eforia (LC₅₀ = 156.24 mg a.i/l) and hexaflumuron (LC₅₀ = 670.32 mg a.i/l). Mixing emamectin benzoate with acetamiprid at the ratio of LC₅₀:LC₅₀and LC₂₅:LC₂₅resulted in synergistic impacts while mix of two other ratios of the same pesticides represented additive influences. The mixture of emamectin benzoate with either hexaflumuron or eforia at all ratios created antagonistic and additive effects, respectively. Mixing emamectin benzoate with either acetamiprid or eforia increased larval esterase activity, however, there was no significant difference between emamectin benzoate in mixture with hexaflumuron and using it alone. Mixing emamectin benzoate with the examined insecticides considerably decreased the larval total protein content. Based on the findings of this work, the mixtures of eforia and acetamiprid with emamectin benzoate represented greater negative effects against 4^th-instars compared to emamectin benzoate alone and the control.

Keywords

mixture effects

emamectin benzoate

acetamiprid

eforia

Tuta absoluta

20.1001.1.22519041.2020.9.4.7.0

Subjects

Chemical Control (all aspects of pesticides)

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