Volume 6, Issue 1 (2017)                   JCP 2017, 6(1): 99-113 | Back to browse issues page

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Saberi E, Alavi S M, Safaie N, Moslemkhany C, Azadvar M. Bacterial pathogens associated with citrus huanglongbing-like symptoms in southern Iran. JCP. 6 (1) :99-113
URL: http://jcp.modares.ac.ir/article-3-10666-en.html
1- Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
2- Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
3- Plant Protection Research Department, South Kerman Agricultural and Natural Resources Research Centre, AREEO, Jiroft, Iran.
Abstract:   (1795 Views)
Huanglongbing (HLB) also known as citrus greening, is a destructive disease of citrus and now, is considered as a new emergence and spread out threat to the Middle East and North Africa (MENA) citrus production. In a survey conducted in southern Iran in 2013-2014, 77 citrus samples exhibiting symptoms of HLB were collected. Single-step and nested polymerase chain reactions (PCR) were employed to determine the presence of the phloem-limited bacterial pathogens ‘Candidatus Liberibacter asiaticus’(CLas), phytoplasma and Spiroplasma citri. Both CLas and phytoplasma were detected in HLB-affected citrus trees as co-infection (7.79%) and single infection (10.38% for phytoplasma and 42.85% for CLas). According to the proposed 16S rDNA-based phytoplasma classification scheme, the HLB-associated phytoplasma from this study was a member of peanut witches’broom (16SrII) phytoplasma group. This is the first report of association of a phytoplasma with HLB in sweet lime in the world and first record of association of CLas with sour orange (Citrus aurantium L.) and sweet lime in Iran.
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Article Type: Full Paper | Subject: Prokaryotic Plant Diseases
Received: 2016/09/22 | Accepted: 2017/04/13 | Published: 2017/06/9

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