Volume 6, Issue 1 (2017)                   JCP 2017, 6(1): 67-77 | Back to browse issues page

‎ 20.1001.1.22519041.2017.6.1.5.1

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rabiei-Motlagh E, Rouhani H, Shokouhifar F, Falahati Rastegar M, Taher P. Molecular identification of formae specialis and racial identity in Iranian strains of Fusarium oxysporum f. sp. lycopersici: detection of avirulence genes. JCP 2017; 6 (1) :67-77
URL: http://jcp.modares.ac.ir/article-3-4485-en.html
Molecular identification of formae specialis and racial identity in Iranian strains of Fusarium oxysporum f. sp. lycopersici: detection of avirulence genes
Elahe Rabiei-Motlagh1 , Hamid Rouhani 1, Farhad Shokouhifar2 , Mahrokh Falahati Rastegar1 , Parissa Taher1
1- Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
2- Plant Science Research Institute, Ferdowsi University of Mashhad, Mashhad, Iran.
Abstract:   (5152 Views)
Fusarium oxysporum f. sp. lycopersici (Fol) is the causal agent of vascular wilt in tomato, an important plant disease in Iran. Four monogenic resistance genes in tomato are used for identification of races of Fol and their corresponding avirulence genes Avr1, Avr2 and Avr3 were identified in pathogen one of which, Avr2, is f.sp. specific. Hence they can serve as reliable markers for racial identity and f.sp discrimination. These markers have been used for strains from other countries except Iran. Furthermore, a point mutation in Avr3 can lead to enhanced virulence of Fol on a susceptible tomato cultivar. To identify forma specialis and racial identity, Avr genes were studied in a collection of Iranian strains. Results revealed that PCR assay is very efficient in distinguishing between non-pathogenic and low virulence strains and in the vast majority of strains, avirulence genotype was consistent with Fol race1. Furthermore, to determine whether allelic variation of Avr3 could separate strains of different degrees of virulence, Avr3 wassequenced in Fol strains with high and low virulence. The resultsrevealed that allelicvariation of Avr3 was not correlated with degree of virulence in Iranian strains.
 
Keywords: Avirulence genotype, effector gene, tomato wilt, molecular detection
Full-Text [PDF 691 kb]   (2767 Downloads)    
Article Type: Full Paper | Subject: Mycology and Fungal Plant Diseases
Received: 2016/08/11 | Accepted: 2016/12/10 | Published: 2017/04/7
Add your comments about this article : Your username or Email:
CAPTCHA
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.