Volume 7, Issue 2 (2018)                   JCP 2018, 7(2): 207-217 | Back to browse issues page

‎ 20.1001.1.22519041.2018.7.2.10.5

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Mahmoudi S B, Saffarian Abbaszadeh M, Abbasi S, Farrokhinejad R. Genetic diversity and pathogenic variability among Cercospora beticola Sacc. isolates causing leaf spot of sugar beet in Iran. JCP 2018; 7 (2) :207-217
URL: http://jcp.modares.ac.ir/article-3-16893-en.html
Genetic diversity and pathogenic variability among Cercospora beticola Sacc. isolates causing leaf spot of sugar beet in Iran
Seyed Bagher Mahmoudi 1, Maryam Saffarian Abbaszadeh2 , Saeed Abbasi3 , Reza Farrokhinejad4
1- Sugar Beet Seed Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.
2- Clemson University, South Carolina, USA.
3- Department of Plant Protection, College of Agriculture, Razi University, Kermanshah, Iran.
4- Department of Plant Protection, College of Agriculture, Shahid Chamran Universty of Ahvaz, Ahvaz, Iran.
Abstract:   (2986 Views)
Cercospora leaf spot caused by Cercospora beticola has a great negative impact on yield and quality of sugar beet. In the present study, pathogenic and genotypic variation of 24 C. beticola isolates collected from different regions of Iran were studied using RFLP of the Internal Transcribed Spacer (ITS-RFLP), and Random Amplified Polymorphic DNA (RAPD-PCR). Pathogenic variability and genotype × isolate interaction were evaluated in greenhouse experiments on five sugar beet cultivars (FD0018, HM1836, Puma, Eudora and Monatuna). All of the 24 isolates tested were found to be pathogenic on the cultivars with significant variation in disease severity. Results of RAPD analysis showed wide DNA polymorphism among the Iranian C. beticola isolates. Restriction pattern of the internal transcribed spacer of rDNA (ITS1-5.8-ITS4) was studied using three restriction endonucleases: EcoR1, Taq1, and Busr1. The length of undigested DNA fragment of all isolates was estimated to be 500bp without rDNA polymorphism after digestion with EcoR1 (280, 270 bp), Taq1 (330 bp) and Busr1 (240, 220, 90 bp). RAPD and ITS-RFLP markers showed the highest level of genetic diversity which confirms the variation in C. beticola detection.
Keywords: Cercospora beticola, genotype × isolate interaction, Pathogenicity, RAPD fingerprinting
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Article Type: Full Paper | Subject: Acarology (Biological Control)
Received: 2017/12/17 | Published: 2018/07/15
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