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Showing 2 results for Sanjarian
Sohrab Moradi, Forough Sanjarian, Naser Safaie, Amir Mousavi, Gholam Reza Bakhshi Khaniki,
Volume 2, Issue 3 (9-2013)
Abstract
One of the important technical obstacles in the study of many filamentous fungi is the development of efficient transformation system. Transformation of filamentous fungi is difficult because they have a cell wall and for most frequently used approaches preparing of protoplast is necessary. Protoplast preparation is batch-dependent and often frustrating work. In this study, a modified method was introduced for gene transfer to the plant pathogenic fungus, Fusarium graminearum, the major causal agent of Fusarium Head Blight disease in small grains. This protocol was based on protoplast-PEG method. Age of mycelia, enzymes exposure time and mycelium/enzyme ratio were optimized for the purpose of protoplast preparation. The outcome showed that the best result for protoplast preparation was obtained when 1.5 × 105 spores were let germinate for 6 h then exposed to 10 ml of enzyme solution for 3 h. The effect of other parameters that might enhance transformation yields including PEG concentration, DNA quantity and number of protoplasts was also examined. The most efficient condition for transformation involved the use of 106 protoplasts, 20 µg DNA and 30% PEG (w/v). In the course of this study, a simple and appropriate modified protocol for transformation of F. graminearum was established. The method introduced is also more economical and faster than other current methods.
Volume 10, Issue 1 (Winter 2019)
Abstract
Aims: Thymus Garden (Thymus vulgaris L.) is one of the economically important plants which is extremely sensitive to oxidative stress and drought stress during germination time. Salicylic acid, as an herbal hormone, plays an important role in increasing plant tolerance to biotic and abiotic stresses. The current study was conducted aiming to increase the plant resistance to environmental stress by increasing its enzymatic and non-enzymatic antioxidant capacity by salicylic acid treatment.
Materials & Methods: In this experimental study, the plant seeds were soaked in 2mM salicylic acid solution a randomized complete block design with three replicates for 16 hours, and they were then planted in pots. Pots were transferred to growth chamber with constant and controlled conditions for 16 hours of light: 8 hours of dark at a temperature of 25°C for 14 days. At the end of the experiment, the growth parameters of plants, germination percentage, phenol content, and the activity of the important antioxidant enzymes, such as superoxide dismutase, catalase, polyphenol oxidase and peroxidase, were measured and compared with the control group.
Findings: Although salicylic acid did not have a significant impact on plant growth, it has led to an effective of antioxidant enzymes in the plant. Moreover, this treatment has increased the antioxidant content of the plant.
Conclusion: Treatment with salicylic acid could result in an increase in Garden Thyme tolerance to stress conditions.
