Showing 8 results for Rashki
Volume 3, Issue 2 (6-2015)
Abstract
TheChah Nimeh reservoirs of Sistan is as one of the main water sources of Zabol City used for drinking, agriculture, industry and livestock purposes. This study aimed to determine the contribution of the natural and concentration factors of heavy metals such as Cd, Pb and Cu in sediment of Chah Nimeh of Sistan. The concentrations of heavy metals in surface sediments were studied in six stations during 2013. To determine the concentration of the heavy metals atomic absorption spectrometer (Contr AA 700) was used. Sequential extraction analysis was applied based on the share of natural and concentration factor sources of heavy metals. Sequential extraction analysis showed that a considerable part of the Pb (63.67%), Cd (55.17%) and Cu (57.17%) were from natural sources. The highest concentrations of Cd and Pb were 0.81 and 5.57 mg kg-1, respectively, obtained in station one and the maximum Cu concentration was observed in station five (37.8 mg kg-1), while the lowest concentration of Cd, Pb and Cu were 0.34, 3.95 and 31.4 mg kg-1, respectively.
Maryam Rashki, Fatemeh Talepour, Asghar Shirvani,
Volume 4, Issue 4 (12-2015)
Abstract
The sub-lethal effect of Metarhizium anisopliae and imidacloprid as well as combination of the two control agents was examined on life table parameters of Myzus persicae on different cultivars of canola under laboratory condition at 25 ± 1 ºC, 85% RH and photoperiod of 16L: 8D. The offspring resulting from fungus-infested adults were placed onto leaf discs in Petri dishes separately to record their development time till they reached to adulthood. The number of nymphs produced by each adult aphid was recorded daily. The intrinsic rate of increase(rm) had no significant differences among the three cultivars, and was nearly similar to the net reproductive rate (R0). The concurrent application of M. anisopliae and imidacloprid significantly shortened the aphid longevity on the RGS003 cv compared to the other cultivars. The type of cultivars had no impact on the finite rate of increase (λ) of green peach aphid in any of the treatments. The values of T showed that there was no significant difference among the treatments. Despite having no significant effect on life table characteristics of M. persicae by most of the treatments, the method would be a favorable procedure to control the aphid by raising the fungal concentration. Conducting such research would be worthwhile since there are no antagonistic interactions between the entomopathogenic fungus and the insecticide.
Volume 9, Issue 3 (Summer 2021)
Abstract
Aims: Nurses are a key component of the treatment team in times of crisis and are currently at the front line of the fight against the COVID-19 pandemic. Since understanding nurses' experiences can help identify the relevant problems, this study aimed to explain nurses' experiences in the coronavirus crisis by content analysis approach.
Participants & Methods: This qualitative study with a conventional content analysis approach conducted purposeful sampling in 2020. Ten nurses who were working in the COVID-19 wards of Amir Al-Momenin Hospital in Zabol were selected. The data collection method was semi-structured interviews. The data analysis process was performed according to the steps proposed by Graneheim and Lundman 2004. The trustworthiness of the data was checked by Lincoin & Guba criteria, and the research's ethical standards were observed.
Findings: Two main themes of nurses and families under the shadow of coronavirus and the dual reaction of nurses to coronavirus crisis, as well as six categories (Nurse's family challenges, Joys and Concerns of nurses, Nurses and care injuries, Nurses and conflicting thought to the profession, Dual care reactions, Dual feeling reactions) and ten subcategories, were extracted.
Conclusion: The results of this study indicate the individual, family, and occupational effects of the corona crisis on nurses, which can affect their care performance in addition to individual life.
Pariya Soltani-Nezhad, Fariba Mehrkhou, Maryam Rashki,
Volume 11, Issue 4 (1-2022)
Abstract
Isolates were identified by molecular and morphological tests, including coleopteran-specific cry genes in the Iranian native Bacillus thuringiensis collection. Spherical and irregular shapes were observed to be the most frequent shapes using Coomassie brilliant blue staining. PCR analysis with universal and specific primer pairs was used to detect coleopteran-specific cry genes such as cry1I, cry3, cry7, cry18, and cry26. All the isolates contained at least one active coleopteran-cry gene, while the most abundant isolates had cry26 and cry18 genes. The patterns of protein size were characterized in addition to their insecticidal activity against third-instar larvae of Tribolium castaneum. Protein profiles produced bands that varied from 14-180 kDa. Four native isolates containing coleopteran-active cry genes displayed higher activity against T. castaneum larvae than B. thuringiensis subspecies galleriae as a reference strain. The median lethal concentration (LC50) of the most pathogenic isolate, PS1078, was 2.72 × 106 spores/ml. Its 16S rDNA gene sequence analysis demonstrated similarity to B. thuringiensiss subspecies galleriae. The characterization of isolates provided useful data for selecting new isolates to expand novel bio-insecticidal products.
Volume 22, Issue 2 (Spring 2019)
Abstract
Aims: The chimeric domain-exchanged streptokinase (SKch) between two sk genes from groups G and A streptococci (SK2aG88 and SK2bALAB49, respectively) was constructed to evaluate the role of SK-domains (α, β, γ) in α2-antipalsmin-resistance variations of SK.
Materials and Methods: In this experimental study, PCR-amplified genes of streptococci (skg, ska) were cloned into pET26b vector to produce pET26-SKG88 and pET26-SKALAB49. For domain exchange, the amplicon containing β and g domains of SK2bALAB49 was replaced for that of the SK2aG88 within pET-SK2aG88 (pET26-SKch; α2aG88β2bALABγ2bALAB). All constructs were confirmed by restriction analyses/agarose-gel electrophoresis and DNA sequencing, transformed into E.coli Rosetta, and induced by IPTG for protein expression. Proteins were purified by Ni-NTA chromatography, quantified by Bradford method, and analyzed by SDS-PAGE/Western blotting assays. The α2-antipalsmin-resistance was measured by S2251 colorimetric assay for plasminogen activation.
Findings: SDS-PAGE and western blotting results indicated the expression of proteins with the size of 47kD. At the highest concentration of α2-antiplasmin, SK2aG88 remained 80% active, whereas the SK2bALAB49 and SKch retained 55% of their activity.
Conclusion: SKch shows similar activity reduction, indicating the minor role of the α domain compared to β and g domains for α2-antipalsmin-resistance.
Volume 22, Issue 3 (Summer 2019)
Abstract
Aims: Premature ovarian failure is a syndrome causing amenorrhea, infertility, and increases gonadotropin levels before age 40. The use of chemotherapy drugs can be one of the reasons that lead to this disorder. The purpose of this study was to evaluate the presence of germ cells markers in mice model of premature ovarian failure following chemotherapy drugs.
Materials and Methods: In this study, 24 mature female mice were used to create a premature ovarian failure model, different amount of cyclophosphamide and busulfan were applied (experimental groups 1 to 5). Bodyweight change, vaginal smear, morphological alternation of ovarian tissue in both experimental and control (without treatment) groups were evaluated and for the best model, hormonal evaluation (FSH, E2), and expression of germline markers (Oct4, Dazl) were examined.
Findings: Since, in the second group estrus cycle disorder, the significant decrease in weight and ovarian reserve (p˂0.05) were observed, compared to the control group, so this group was chosen as the best model. An increase in FSH level and reduction in estradiol level in the second group, compared with the control group (p˂0.05), confirmed creation of the POF model. Also, genes expression of Oct-4 and Dazl showed an increase (p˂0.05) in the second group compared with the control one.
Conclusion: The presence of germ cells markers in a mouse model of premature ovarian failure following the use of chemotherapy drugs can be a new hope in the treatment of infertility in cancer patients after chemotherapy.
Volume 23, Issue 1 (Winter 2020)
Abstract
Interleukin 15 (IL-15) is a cytokine that, due to its physiological activity, can play a role in anti-cancer therapies. It shows positive effect on Natural Killer cells differentiation, proliferation, activation, and surveillance and also on surveillance of memory CD8+ T cells. However, the expression and purification yield of recombinant IL-15 is low. So, it worth improving the production conditions of this useful protein. Therefore in the present study, cloning and expression of human IL-15 are reported in E. coli, strain Rosetta (DE3) which is different from previous bacterial hosts BL21 strain. This strain (Rosetta DE3) has the potential to use codons rarely used by bacteria and consequently, the expressed protein can be more similar to the human protein. First, the human IL-15 coding sequence was synthetized, and then the sequence was cloned into pET28a plasmid. Confirming the accuracy of the final construct was done by colony PCR, restriction analysis (which was done using BamHI and XhoI restriction enzymes and the expected 391bp band was observed) and sequencing. Then, the recombinant construct was transformed into competent E. coli Rosetta (DE3) bacteria. Expression was done in OD600 of 0.6 in the presence of 1mM Isopropyl ß-D-1-thiogalactopyranoside (IPTG). Protein characterization was done by SDS-PAGE and then by western blotting using a specific commercial anti-IL-15 antibody. The 15kDa band on the gel and blot, showed the presence of IL-15. Densitometry by Fiji software determined 37% production yield. It is expected a suitable function from this produced recombinant cytokine due to expression in E. coli Rosetta (DE3) in future studies.
Volume 24, Issue 1 (Spring 2021)
Abstract
Measles virus, negative-strand RNA viruses, has been known as an ideal candidate in oncolytic virotherapy. Recombinant measles virus can encode genes of interests for reaching several aims. Replication efficiency of oncolytic virus in tumoral cells is a key parameter in efficient tumor eradication. Products of P gene (P/V/C) support measle virus to circumvent IFN 1 as the main response of innate immune system against viruses. But vaccine strains used in oncolytic therapy studies comprise several mutations in their P gene sequences. These mutations affect replication efficacy which cause attenuation of measles strains applicable in vaccination. So, arming vaccine strains with the wild type P gene is helpful to reach high virus titer. Here at this study, we have expanded a protocol with details for engineering and efficient recovery of measles virus for different aims.
