Volume 6, Issue 1 (2017)                   JCP 2017, 6(1): 35-44 | Back to browse issues page

XML Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Chehri K, Hasani S M. Identification of Aspergillus flavus and aflatoxins contamination in inflorescences of wild grasses in Iran. JCP. 6 (1) :35-44
URL: http://jcp.modares.ac.ir/article-3-7884-en.html
1- Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
2- Department of Biology, Faculty of Science, Islamic Azad University, Yasuj Branch, Iran.
Abstract:   (4250 Views)
Wild grasses are the most important primary feedstuffs which are susceptible to contamination with toxigenic fungi belonging to Aspergillus spp. In order to explore diversity of Aspergillus species associated with the inflorescences of gramineous weeds, infected inflorescences were collected from wild grasses in western parts of Iran. Fifty-six Aspergillus isolates were obtained from all diseased spikes and based on morphological features identified as 4 species i.e. Aspergillus niger (26) followed by Aspergillus flavus (24), Aspergillus fumigatus (4), andAspergillusjaponicus (2). The identification of A. flavus was confirmed using species specific primers of AFLA-F/AFLA-R by producing amplicons about 413 bp. In this study, aflatoxins (AFs) contamination of wild grasses was evaluated by enzyme linked immunosorbent assay (ELISA). Natural occurrence of AFs could be detected in 24 samples ranging from 0.63-134.86 μg/kg. The highest AFT levels were detected in samples from Ravansar, Bisetoon, Mahidasht, and Sarpol Zehab (up to 50 μg/kg), which is more than the recommended limits by European :union: standard and National Standard of Iran (20 µg/kg for animal feed).
Full-Text [PDF 588 kb]   (1993 Downloads)    
Article Type: Full Paper | Subject: Mycology and Fungal Plant Diseases
Received: 2016/02/15 | Accepted: 2016/11/28 | Published: 2017/01/26

Add your comments about this article : Your username or Email:
CAPTCHA