Tarbiat Modares University Journal of Crop Protection 2251-9041 14 4 2025 10 17 Phytotoxic potential of actinomycetes isolated from Mimosa pudica and Oryza sativa 389 403 27855 10.48311/jcp.2025.76101.0 EN Alimuddin Ali Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Makassar, Indonesia 90224. Biotechnology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Makassar, Indonesia 90222. Muhammad Junda Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Makassar, Indonesia 90224. Arni Putri Merdeka Wati Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Makassar, Indonesia 90224. Oslan Jumadi Biology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Makassar, Indonesia 90224. Biotechnology Study Program, Faculty of Mathematics and Natural Sciences, Universitas Negeri Makassar, Makassar, Indonesia 90222. Herlina Rante Laboratory of Pharmacy Microbiology, Faculty of Pharmacy, Hasanuddin University, Jl. Perintis Kemerdekaan Km 10, Makassar, Indonesia 90245. Journal Article 2023 10 04 This study aims to evaluate the phytotoxic activity produced by actinomycetes isolated from rhizosphere and endophytic plants. The study was conducted using 12 actinomycete strains recovered from various plants in South Sulawesi, Indonesia. The phytotoxin from the culture filtrate of fermentation broth of all strains was bioassayed on filter paper against seed germination of Mimosa pudica and Oryza sativa. The results showed that the actinomycetes culture filtrate of strain AAE05 and AAE16 has an 80% and 50% reduction in mimosa and paddy seed germination, respectively. Meanwhile, strain AAE16 reduced both test plants in the pre-emergence condition. Furthermore, the AAE16 strain’s 16S rRNA sequence was highly related to that of the <span lang="ZH-CN" style="font-size: 10.0pt; font-family: 'Times New Roman',serif; mso-fareast-font-family: 'Times New Roman'; mso-bidi-font-family: 'B Lotus'; mso-ansi-language: ZH-CN; mso-fareast-language: ZH-CN;">Streptomyces pseudogriseolus strain NRRL B-3288. The phytotoxic activity of culture filtrates from AAE16 was also evident in germinated seedlings of both plants, as indicated by leaf curling, wilting, and burning in the post-emergence period. These results suggested that actinomycetes are a promising source of phytotoxic agents. Therefore, further investigations are recommended to explore these substances for structure elucidation, which may prove to be promising bioherbicide agents.

https://jcp.modares.ac.ir/article_27855_6c766ed2f0289e20a8097cfdba95f311.pdf