Tarbiat Modares University Journal of Crop Protection 2251-9041 8 4 2019 08 01 Detection and molecular characterization of tomato big bud disease in Qazvin province 379 388 1443 10.48311/jcp.2019.1443 EN Abbas Davoodi Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran. Naser Panjekeh Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran. Kobra Moslemkhani Seed and Plant Certification and Registration Institute, AREEO, Karaj, Iran. Abdolhossein Taheri Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran. Journal Article Some samples were collected from tomato fields in Qazvin from tomato plants with big bud symptoms such as plant droop and purplish vein under the leaf, enlarged and sac-like pistils and malformed buds. DNA was extracted from the veins and vascular tissues of the plant with CTAB-based methods. In symptomatic plants, DNA fragments of 1800 and 1200bp were amplified by PCR using P1/P7, R16F2n/R16R2 primers. Restriction fragment length polymorphism (RFLP) analysis of nested R16F2n/R16R2 primed PCR product (1200bp) showed that the tomato big bud phytoplasma from Qazvin (TOM-Qazvin) is a member of clover proliferation (16SrVI). Phylogenetic analysis of 16SrRNA and putative restriction site analysis of the R16F2n/R16R2 primed sequence classified TOM phytoplasma in clover proliferation (16SrVI) group and belonged to subgroup 16SrVI-A. Virtual RFLP by using 1200bp sequencing of 16SRNA and 17 restriction enzymes confirmed that TOM-Qazvin belonged to the subgroup 16SrVI-A and16SrVI group. To our knowledge, this is the first report of tomato big bud disease in Qazvin province. https://jcp.modares.ac.ir/article_1443_8fb5f8be2aa9d6c64a04e3ab9f63feee.pdf