Tarbiat Modares University Journal of Crop Protection 2251-9041 4 5 2015 12 01 Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects 675 690 1249 10.48311/jcp.2015.1249 EN Masoumeh Kordi Biotechnology Research Department, Shahrood University, Shahrood, Iran. Naser Farrokhi Department of Biotechnology Engineering, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran. Abolfazl Masoudi Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University, Sahrood, Iran. Ali Derakhshan Shadmehri Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University, Sahrood, Iran. Shahrokh Gharanjik Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University, Sahrood, Iran. Journal Article <em>Beauveria </em>spp. as entomopathogenic fungi have extremely diverse insect host ranges.Here, a comparative transcript analysis of a <em>Bassiasin I</em> (<em>BSNI</em>), a <em>Dipeptidyl peptidase </em>(<em>DPP</em>), a <em>Cytochrom P450 monooxygenase</em> (<em>CYP52</em>), a <em>Mitogen activated protein kinase</em> (<em>MAPK</em>), and a <em>Neuronal calcium sensor 1</em> (<em>NCS</em>) in two isolates of <em>Beauveria</em> <em>bassiana</em>., with low and high virulence, were carried out. For this, induction media, containing isolated cuticles from four insects, and a check medium lacking any cuticular extract were prepared and isolates were allowed to grow on them. Interestingly, up-regulation of <em>BSNI</em> and <em>DPP</em> in induction media was noted in comparison to the check, following normalization with <em>γ-ACTIN</em>. <em>CYP52</em> was up-regulated in synthetic medium in comparison to cuticular extract. <em>MAPK</em> expression was greater in most virulent isolate once compared with the low virulent isolate. The expression of <em>MAPK</em> was greater in the presence of <em>Caliptamus italicus</em> cuticles and synthetic medium as opposed to others. <em>NCS</em> expression remained on changed in all media tested and can be suggested as a reference gene in transcriptomics of <em>Beauveria</em> spp. genes in future. https://jcp.modares.ac.ir/article_1249_2ba8698b79439589fdd2b0f7218d8b07.pdf