Journal of Crop Protection
Journal of Crop Protection
JCP
Agriculture
http://jcp.modares.ac.ir
1
admin
2251-9041
2251-905X
en
jalali
1395
12
1
gregorian
2017
3
1
6
1
online
1
fulltext
en
Identification of Aspergillus flavus and aflatoxins contamination in inflorescences of wild grasses in Iran
Wild grasses are the most important primary feedstuffs which are susceptible to contamination with toxigenic fungi belonging to <em>Aspergillus</em> spp. In order to explore diversity of <em>Aspergillus</em> species associated with the inflorescences of gramineous weeds, infected inflorescences were collected from wild grasses in western parts of Iran. Fifty-six <em>Aspergillus</em> isolates were obtained from all diseased spikes and based on morphological features identified as 4 species i.e<em>. </em><em>Aspergillus</em><em> niger</em> (26) followed by <em>Aspergillus</em><em> flavus </em>(24)<em>, </em><em>Aspergillus</em><em> fumigatus </em>(4)<em>, </em>and<em>Aspergillus</em><em>japonicus </em>(2). The identification of <em>A. flavus</em> was confirmed using species specific primers of AFLA-F/AFLA-R by producing amplicons about 413 bp. In this study, aflatoxins (AFs) contamination of wild grasses was evaluated by enzyme linked immunosorbent assay (ELISA). Natural occurrence of AFs could be detected in 24 samples ranging from 0.63-134.86 μg/kg. The highest AFT levels were detected in samples from Ravansar, Bisetoon, Mahidasht, and Sarpol Zehab (up to 50 μg/kg), which is more than the recommended limits by European :union: standard and National Standard of Iran (20 µg/kg for animal feed).
Aflatoxins,species specific primers,ELISA
35
44
http://jcp.modares.ac.ir/browse.php?a_code=A-3-15845-1&slc_lang=en&sid=3
Khosrow
Chehri
Khosrow
Chehri
100319475328460067903
100319475328460067903
Yes
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran.
Seyyed Meqdad
Hasani
Seyyed Meqdad
Hasani
100319475328460067904
100319475328460067904
No
Department of Biology, Faculty of Science, Islamic Azad University, Yasuj Branch, Iran.
Department of Biology, Faculty of Science, Islamic Azad University, Yasuj Branch, Iran.