Journal of Crop Protection
Journal of Crop Protection
JCP
Agriculture
http://jcp.modares.ac.ir
1
admin
2251-9041
2251-905X
en
jalali
1394
9
1
gregorian
2015
12
1
4
4
online
1
fulltext
en
Elimination of Bean yellow mosaic virus through thermotherapy combined with meristem-tip culture in gladiolus corms
<em>Bean yellow mosaic virus</em> (BYMV, <em>Potyvirus</em>, <em>Potyviridae</em>) causes serious disease in <em>Gladiolus</em> spp. In this work, the possibility of obtaining BYMV free plant material from virus infected gladiolus corms was studied. Thermotherapy, meristem-tip culture and combination of both techniques on infected corms/meristem-tip explants (0.5–1 mm in length) resulted in BYMV elimination up to 15.38, 78.04 and 86.66%, respectively, as determined by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) and reverse transcription-polymerase chain reaction (IC-RT-PCR). Individual virus-free shoots readily rooted in vitro and were transferred to corm formation medium. The results showed that thermotherapy promotes the survival rate of explants during meristem-tip culture steps (except regeneration step) and also plantlet acclimatization. Statistical analysis showed that the BYMV elimination in gladiolus corms was significantly (P ≤ 0.01) affected by thermotherapy treatment of infected corms. Thermotherapy combined with meristem culture can greatly improve BYMV elimination efficiency from infected gladiolus corms, resulting in the production of BYMV free gladiolus plants.
BYMV,Gladiolus,Meristem-tip culture,thermotherapy,Virus free corm
533
543
http://jcp.modares.ac.ir/browse.php?a_code=A-3-1000-5263&slc_lang=en&sid=3
Parisa
Sharifi Nezamabad
Parisa
Sharifi Nezamabad
100319475328460057396
100319475328460057396
No
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University of Tehran, Karaj, Iran.
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University of Tehran, Karaj, Iran.
Mina
Koohi Habibi
Mina
Koohi Habibi
100319475328460057397
100319475328460057397
No
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University of Tehran, Karaj, Iran.
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University of Tehran, Karaj, Iran.
Akbar
Dizadji
Akbar
Dizadji
100319475328460057398
100319475328460057398
Yes
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University of Tehran, Karaj, Iran.
Department of Plant Protection, Faculty of Agricultural Sciences and Engineering, University of Tehran, Karaj, Iran.
Siamak
Kalantari
Siamak
Kalantari
100319475328460057369
100319475328460057369
No
Department of Horticulture Science, Faculty of Agricultural Sciences and Engineering, University of Tehran, Karaj, Iran.
Department of Horticulture Science, Faculty of Agricultural Sciences and Engineering, University of Tehran, Karaj, Iran.