Journal of Crop Protection
Journal of Crop Protection
JCP
Agriculture
http://jcp.modares.ac.ir
1
admin
2251-9041
2251-905X
en
jalali
1398
5
1
gregorian
2019
8
1
8
4
online
1
fulltext
en
Characterization and genetic variability of coat protein gene of Apple chlorotic leaf spot virus isolates from apple in Kurdistan and western Azarbaijan Provinces, Iran
پژوهشی اصیل
Original Research
In this study, 174 leaf samples from main apple growing areas in Kurdistan and western Azarbaijan Provinces were examined for infection by <em>Apple chlorotic leaf spot virus</em> (ACLSV). The result of RT-PCR showed that 17 samples (9.7%) were infected by ACLSV in both provinces. Subsequently, the genetic structure of ACLSV was determined based on the full coat protein (CP) sequences of eight isolates from this study and those recently published from Iran and worldwide. In phylogenetic analysis, ACLSV isolates from apple clustered into three known phylogenetic types; B6, P205, and SHZ, which are characterized by three amino acids at positions of 40, 75 and 79. The new Iranian ACLSV isolates showed the CP amino acid sequence of Ser<sup>40</sup>-Tyr<sup>75</sup>-Glu<sup>79 </sup>which placed them with the B6 type. Further analyses showed that the Iranian ACLSV isolates (the new and previously published isolates) had lower genetic variability compared to isolates from other countries except for Brazilian population. Most of the CP codons were under negative selection (dN/dS < 1) except for one codon at position 92 which was under positive selection. This study suggests that negative selection was most important evolutionary factor driving the genetic evolution of ACLSV.<br>
apple, negative selection, phylogenetic analysis, ACLSV
491
500
http://jcp.modares.ac.ir/browse.php?a_code=A-10-48081-2&slc_lang=en&sid=3
Kamal
Shujaei
k.shujayi.fid@gmail.com
1003194753284600141058
1003194753284600141058
No
Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.
Mahdi
Azaryar
mahdi_a1991@yahoo.com
1003194753284600141059
1003194753284600141059
No
Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.
Mohammad
Hajizadeh
hajizadeh2003@yahoo.com
1003194753284600141060
0000000207743523
Yes
Department of Plant Protection, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran.