Journal of Crop Protection
Journal of Crop Protection
JCP
Agriculture
http://jcp.modares.ac.ir
1
admin
2251-9041
2251-905X
en
jalali
1393
3
1
gregorian
2014
6
1
3
2
online
1
fulltext
en
Biochemical characterization of digestive β-glucosidase from midgut of Leiptinotarsa decemlineata (Coleoptera: Chrysomelidae)
The Colorado potato beetle, <em>Leptinotarsa decemlineata </em>Say (Col.: Chrysomelidae) is an important pest of potato worldwide and study of its glucosidases is an important step to develop appropriate pest control strategies. In this study, some biochemical aspects of β-glucosidase in the Colorado potato beetle were determined. The results showed that β-glucosidase activity in the midgut of adults was 6.68 Umg<sup>-1</sup>. Maximum activity of midgut β-glucosidase occurred at pH 4 to pH 5.5; however, the enzyme is active at pH 3 to pH 7 more than 50% of its relative activity. The enzyme was stable at pH 3 to pH 8 for 2 and 8 hours incubation time. According to the results, optimal temperature for the enzyme activity was 50 °Cand its stability significantly was reduced at 50 °C during 1 to 8 days incubation time. The enzyme activity decreased with the addition of different concentrations of MgCl<sub>2</sub>, urea, Tris and CaCl<sub>2</sub>. Enzyme activity was highly decreased at low concentrations of SDS (1 mM).
Biochemistry,midgut,β-glucosidase,Leptinotarsa decemlineata
181
189
http://jcp.modares.ac.ir/browse.php?a_code=A-3-1000-146&slc_lang=en&sid=3
Fahimeh
Dehghanikhah
Fahimeh
Dehghanikhah
100319475328460050462
100319475328460050462
No
Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Majid
Kazzazi
Majid
Kazzazi
100319475328460050461
100319475328460050461
Yes
Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Hossein
Madadi
Hossein
Madadi
100319475328460050463
100319475328460050463
No
Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Department of Plant Protection, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran.
Vahid
Hossein naveh
Vahid
Hossein naveh
100319475328460050464
100319475328460050464
No
Department of Plant Protection, College of Agriculture, University of Tehran; Karaj, Iran.
Department of Plant Protection, College of Agriculture, University of Tehran; Karaj, Iran.