Journal of Crop Protection
Journal of Crop Protection
JCP
Agriculture
http://jcp.modares.ac.ir
1
admin
2251-9041
2251-905X
en
jalali
1394
9
1
gregorian
2015
12
1
4
4
online
1
fulltext
en
Genetic diversity of Cytospora schulzeri isolates using RAPD-PCR and MP-PCR markers on Apples of Semirom Region of Iran
Genetic diversity relationships of 50 isolates of <em>Cytospora schulzeri</em> on apple from different parts of the Semirom region were analyzed using 15 polymerase chain reaction (PCR) based markers, 7 random amplified polymorphic DNAs (RAPDs) and 8 Microsatellite primed polymerase chain reaction (MP-PCR). Using 7 selected RAPD primers 113 bands were generated, of which 81 bands were polymorphic (71.7%), with an average of 11.57 polymorphic fragments per primer, and with 8 selected MP-PCR primers 107 amplified bands were observed with 78 polymorphic bands (72.3%), with an average of 9.75 polymorphic fragments per primer. In RAPD marker, number of polymorphic bands varied from 8 (241) to 15 (230, 238, OPA13) with an average of 11.57 per primer and which varied in size from 200 to 3750 bp. Percentage of polymorphism ranged from 64% (203 and 232) to a maximum of 83% (238). In MP-PCR marker, number of polymorphic bands varied from 6 (CAG) to 12 (GTG and ATG) with an average of 9.75 per primer and which varied in size from 200 to 3500 bp. Percentage of polymorphism ranged from 54% (CAG) to a maximum of 81% (ACTG). By combining markers, a total of 220 bands were detected, of which 159 bands (72%) were polymorphic and produced on an average 10.6 polymorphic bands per primer. The results showed that both markers were suitable for the detection of genetic polymorphism among apple <em>C. schulzeri </em>isolates. Estimated genetic relationship using similarity co-efficient (Jaccard’s) values between different pair of accessions varied from 0.54 to 0.89 in RAPD, 0.62 to 0.89 in MP-PCR and 0.62 to 0.87 with combined markers based similarities. High cophenetic correlation between the similarity matrix and corresponding dendrogram was obtained by RAPD + MP-PCR marker (r = 0.81). Cluster analysis of the data using UPGMA based on Jaccard´s similarity coefficient, divided the isolates into six groups, showing a high genetic diversity among populations of <em>C. schulzeri</em>.
Apple,cytospora,genetic diversity,MP-PCR,RAPD
441
452
http://jcp.modares.ac.ir/browse.php?a_code=A-3-1000-1209&slc_lang=en&sid=3
Mehdi
Mehrabi
Mehdi
Mehrabi
100319475328460057866
100319475328460057866
No
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Ebrahim
Mohammadi Goltapeh
Ebrahim
Mohammadi Goltapeh
100319475328460057865
100319475328460057865
Yes
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
Khalil
Berdi Fotouhifar
Khalil
Berdi Fotouhifar
100319475328460057867
100319475328460057867
No
Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.