Journal of Crop Protection
Journal of Crop Protection
JCP
Agriculture
http://jcp.modares.ac.ir
1
admin
2251-9041
2251-905X
en
jalali
1396
12
1
gregorian
2018
3
1
7
1
online
1
fulltext
en
The phenotypic and genotypic characteristics of causal agents of potato bacterial soft rot in Ardabil province of Iran
Bacterial soft rot causes severe damage to potatoes and is responsible for considerable economic losses in potato crop during both growing season and storage period. The most common causal agent of potato soft rot worldwide is <em>Pectobacterium carotovorum </em>(Pc); however in Iran, two other species: <em>P. atrosepticum</em> and <em>P</em>. <em>wasabiae</em> have been reported. Identification and assessment of genetic variation in pathogen populations are very important to understanding taxonomy, epidemiology, and management of a pathogen. In this study, potato stems and tubers showing soft rot symptoms along with their surrounding soil were collected from fields and seed storages of Ardabil province. A total of 33 pectolytic bacterial strains were isolated on nutrient agar and eosin methylene blue culture media. The isolated strains and five standard strains belonging to <em>P<span dir="RTL">.</span> atrosepticum, P. carotovorum</em> and <em>Dickeya dianthicola</em> were studied. The strains were identified as <em>P<span dir="RTL">.</span> carotovorum </em>subsp.<em> carotovorum</em> on the basis of phenotypic characteristics including: gram-negative, facultative anaerobic, soft rot production on potato slices, growth at 37 °C, and inability to produce acid from α-methyl-D-glucoside and also molecular identification using species-specific primers. Based on Y1/Y2 and ExpccF/ExpccR primers, the expected amplicons (434 and 550 bp fragments, respectively) were obtained for all strains and the standard strains belonging to<em> P. carotovorum. </em>According to rep-PCR and cluster analysis using UPGMA and NTSYS 2.1 software, the selected strains were categorized into two main groups and four subgroups. Rep-PCR indicated different levels of genetic heterogeneity among Pcc strains, however, no clear correlation was found between clustering and the geographical origin of the strains.
Pectobacterium carotovorum, rep-PCR, species-specific primers
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119
http://jcp.modares.ac.ir/browse.php?a_code=A-3-13887-1&slc_lang=en&sid=3
Elham
Eshghpour
100319475328460082617
100319475328460082617
No
Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Department of Plant Protection, Faculty of Agriculture, University of Zanjan,45371-38791, Zanjan,Iran
Fatemeh
Shahryari
100319475328460082618
100319475328460082618
Yes
Department of Plant Protection, Faculty of Agriculture, University of Zanjan, Zanjan, Iran.
Department of Plant Protection, Faculty of Agriculture, University of Zanjan,45371-38791, Zanjan,Iran
Abolghasem
Ghasemi
100319475328460082619
100319475328460082619
No
Plant Diseases Department, Iranian Research Institute of Plant Protection, Tehran, Iran.
Plant Diseases Department, Iranian Research Institute of Plant Protection, 19395-1454, Tehran, Iran