TY - JOUR T1 - Molecular characterization of a 16SrIX phytoplasma associated with Convolvulus glomeratus witches' broom and with an insect vector in Iran TT -  خصوصیات مولکولی فیتوپلاسمای 16SrIX مرتبط با بیماری جاروک پیچک Convolvulus glomeratus و ناقل آن در ایران  JF - mdrsjrns JO - mdrsjrns VL - 7 IS - 4 UR - http://jcp.modares.ac.ir/article-3-21282-en.html Y1 - 2018 SP - 387 EP - 393 KW - 16SrIX KW - Convolvulus glomeratus KW - Orosius albicinctus KW - Bandar Abbas KW - witches' broom N2 - During a survey in 2016, Convolvulus glomeratus showing symptoms resembling those caused by phytoplasmas were observed in Bandar Abbas, Hormozgan province, Iran. These plants were examined for phytoplasma presence by nested-PCR assays using universal primer pair P1/P7 followed by R16F2n/R16R2. All the infected plants indicated positive results using universal primer pairs of P1/P7 followed by R16F2n/R16R2. Results of phylogenetic and virtual RFLP analyses of the 16S rRNA gene sequences revealed that the phytoplasma associated with Convolvulus glomeratus witches' broom (CgWB) was a strain of 'Candidatus Phytoplasma phoenicium'. The phytoplasma was successfully transmitted to healthy plants by leafhopper, Orosius albicinctus (Distant) which account as a vector of this phytoplasma. This is the first report on the presence of ‘Candidatus Phytoplasma phoenicium’ (phytoplasma group 16SrIX-J) in C. glomeratus and its insect vector in Iran. M3 ER -