RT - Journal Article T1 - Detection and molecular characterization of tomato big bud disease in Qazvin province JF - mdrsjrns YR - 2019 JO - mdrsjrns VO - 8 IS - 4 UR - http://jcp.modares.ac.ir/article-3-30554-en.html SP - 379 EP - 388 K1 - big bud K1 - iphyclassifier K1 - RFLP K1 - tomato K1 - phytoplasma AB - Some samples were collected from tomato fields in Qazvin from tomato plants with big bud symptoms such as plant droop and purplish vein under the leaf, enlarged and sac-like pistils and malformed buds. DNA was extracted from the veins and vascular tissues of the plant with CTAB-based methods. In symptomatic plants, DNA fragments of 1800 and 1200bp were amplified by PCR using P1/P7, R16F2n/R16R2 primers. Restriction fragment length polymorphism (RFLP) analysis of nested R16F2n/R16R2 primed PCR product (1200bp) showed that the tomato big bud phytoplasma from Qazvin (TOM-Qazvin) is a member of clover proliferation (16SrVI). Phylogenetic analysis of 16SrRNA and putative restriction site analysis of the R16F2n/R16R2 primed sequence classified TOM phytoplasma in clover proliferation (16SrVI) group and belonged to subgroup 16SrVI-A. Virtual RFLP by using 1200bp sequencing of 16SRNA and 17 restriction enzymes confirmed that TOM-Qazvin belonged to the subgroup 16SrVI-A and16SrVI group. To our knowledge, this is the first report of tomato big bud disease in Qazvin province. LA eng UL http://jcp.modares.ac.ir/article-3-30554-en.html M3 ER -