Volume 4, Issue 5 (2015)
JCP 2015, 4(5): 675-690 |
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Masoumeh Kordi1 
, Naser Farrokhi 2, Abolfazl Masoudi3 , Ali Derakhshan Shadmehri3 , Shahrokh Gharanjik3
, Naser Farrokhi 2, Abolfazl Masoudi3 , Ali Derakhshan Shadmehri3 , Shahrokh Gharanjik3
1- Biotechnology Research Department, Shahrood University, Shahrood, Iran.
2- Department of Biotechnology Engineering, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran.
3- Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University, Sahrood, Iran.
2- Department of Biotechnology Engineering, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran.
3- Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University, Sahrood, Iran.
Abstract: (4066 Views)
Beauveria spp. as entomopathogenic fungi have extremely diverse insect host ranges.Here, a comparative transcript analysis of a Bassiasin I (BSNI), a Dipeptidyl peptidase (DPP), a Cytochrom P450 monooxygenase (CYP52), a Mitogen activated protein kinase (MAPK), and a Neuronal calcium sensor 1 (NCS) in two isolates of Beauveria bassiana., with low and high virulence, were carried out. For this, induction media, containing isolated cuticles from four insects, and a check medium lacking any cuticular extract were prepared and isolates were allowed to grow on them. Interestingly, up-regulation of BSNI and DPP in induction media was noted in comparison to the check, following normalization with γ-ACTIN. CYP52 was up-regulated in synthetic medium in comparison to cuticular extract. MAPK expression was greater in most virulent isolate once compared with the low virulent isolate. The expression of MAPK was greater in the presence of Caliptamus italicus cuticles and synthetic medium as opposed to others. NCS expression remained on changed in all media tested and can be suggested as a reference gene in transcriptomics of Beauveria spp. genes in future.
Keywords: Bassiasin I, Dipeptidyl peptidase, Cytochrome P450 monooxygenase, Mitogen activated protein kinase, Neuronal calcium sensor
Article Type: Full Paper |
Subject:
Insect Biological Control
Received: 2015/04/14 | Accepted: 2015/08/24 | Published: 2015/11/1
Received: 2015/04/14 | Accepted: 2015/08/24 | Published: 2015/11/1
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