Volume 1, Issue 2 (2012)                   JCP 2012, 1(2): 97-105 | Back to browse issues page

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Ahmadi F, Khani A, Ghadamyari M. Some properties of α-amylase in the digestive system and head glands of Cryptolaemus montrouzieri (Coleoptera: Coccinellidae). JCP. 1 (2) :97-105
URL: http://journals.modares.ac.ir/article-3-2185-en.html
1- Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran.
2- Department of Plant Protection, Faculty of Agriculture, University of Zabol, Zabol, Iran. Tel. and Fax: +98-542-2242501; E-mail address: abbkhani@yahoo.com
3- Department of Plant Protection, Faculty of Agricultural Science, University of Guilan, Rasht, Iran.
Abstract:   (2644 Views)
Biochemical characteristics of α-amylase in the digestive system and head glands of Cryptolaemus montrouzieri, a key predator of citrus mealybug, Planococcus citri (Pseudococcidae), were studied. The major isoform of α-amylase with the same molecular weight was detected in both gut and head glands loaded on polyacrylamide-starch gel electrophoresis. Moreover, a minor band with much lower intensity was observed in zymogram analysis of gut. Results showed that the specific activity of α-amylase from head glands (0.89 ± 0.02 μmol/min/mg protein) was significantly more than that of digestive system (0.76 ± 0.01 μmol/min/mg protein) in common condition (temperature equal to 25 ± 1 oC). The optimal pH and temperature for α-amylases activity were determined to be nearly 4 and 50°C in digestive system and 6 and 60 °C in head glands, respectively. EDTA (Ethylenediamine tetra acetic acid), Mg2+, Na+, Co2+, Fe2+ and Ca2+ inhibited the enzyme activity but Ba2+, Zn2+, Hg+ and K+ enhanced enzyme activity in digestive system. EDTA and all tested metal ions except Ba2+ inhibited the enzyme activity of head glands. Detectable levels of α-amylase activity in the insect reflect adaptation of the coccinellid for using starch granules or sugars (honeydew; sugary excreta of homopterans; and nectar) as a source of food in addition to predatory habits.
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Received: 2012/02/11 | Accepted: 2012/05/21 | Published: 2012/05/29

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