Volume 7, Issue 4 (2018)                   JCP 2018, 7(4): 387-393 | Back to browse issues page

‎ 20.1001.1.22519041.2018.7.4.6.5

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Nikooei M, Hemmati C. Molecular characterization of a 16SrIX phytoplasma associated with Convolvulus glomeratus witches' broom and with an insect vector in Iran. JCP 2018; 7 (4) :387-393
URL: http://jcp.modares.ac.ir/article-3-21282-en.html
Molecular characterization of a 16SrIX phytoplasma associated with Convolvulus glomeratus witches' broom and with an insect vector in Iran
Mehrnoosh Nikooei1 , Chamran Hemmati 2
1- Department of Agriculture, Minab Higher Education Center, University of Hormozgan, Bandar Abbas, Iran.
2- Plant Protection Research Group, University of Hormozgan, Bandar Abbas, Iran. , chamran.hemmati@hormozgan.ac.ir
Abstract:   (4875 Views)
During a survey in 2016, Convolvulus glomeratus showing symptoms resembling those caused by phytoplasmas were observed in Bandar Abbas, Hormozgan province, Iran. These plants were examined for phytoplasma presence by nested-PCR assays using universal primer pair P1/P7 followed by R16F2n/R16R2. All the infected plants indicated positive results using universal primer pairs of P1/P7 followed by R16F2n/R16R2. Results of phylogenetic and virtual RFLP analyses of the 16S rRNA gene sequences revealed that the phytoplasma associated with Convolvulus glomeratus witches' broom (CgWB) was a strain of 'Candidatus Phytoplasma phoenicium'. The phytoplasma was successfully transmitted to healthy plants by leafhopper, Orosius albicinctus (Distant) which account as a vector of this phytoplasma. This is the first report on the presence of ‘Candidatus Phytoplasma phoenicium’ (phytoplasma group 16SrIX-J) in C. glomeratus and its insect vector in Iran.
 
Keywords: 16SrIX, Convolvulus glomeratus, Orosius albicinctus, Bandar Abbas, witches' broom
Full-Text [PDF 752 kb]   (2087 Downloads)    
Article Type: Brief Communication | Subject: Prokaryotic Plant Diseases
Received: 2018/05/24 | Accepted: 2018/10/10 | Published: 2018/09/15
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.